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Quorum sensing inhibition and tobramycin acceleration in Chromobacterium violaceum by two natural cinnamic acid derivatives.
Applied Microbiology and Biotechnology ( IF 5 ) Pub Date : 2020-04-04 , DOI: 10.1007/s00253-020-10593-0 Wei-Jia Cheng 1 , Jin-Wei Zhou 2 , Ping-Ping Zhang 1 , Huai-Zhi Luo 3 , Shi Tang 1 , Jun-Jian Li 1 , Shi-Ming Deng 1 , Ai-Qun Jia 1, 3
Applied Microbiology and Biotechnology ( IF 5 ) Pub Date : 2020-04-04 , DOI: 10.1007/s00253-020-10593-0 Wei-Jia Cheng 1 , Jin-Wei Zhou 2 , Ping-Ping Zhang 1 , Huai-Zhi Luo 3 , Shi Tang 1 , Jun-Jian Li 1 , Shi-Ming Deng 1 , Ai-Qun Jia 1, 3
Affiliation
Chromobacterium violaceum, one free-living Gram-negative bacterium, is abundantly presented in tropics and sub-tropics soil and aquatic environment; it is also an opportunistic human pathogen. Here, two cinnamic acid derivatives, i.e., 4-dimethylaminocinnamic acid (DCA) and 4-methoxycinnamic acid (MCA), were identified as potential quorum sensing (QS) and biofilm inhibitors in C. violaceum ATCC12472. Both DCA (100 μg/mL) and MCA (200 μg/mL) inhibited the levels of N-decanoyl-homoserine lactone (C10-HSL) and reduced the production of certain virulence factors in C. violaceum, including violacein, hemolysin, and chitinase. Metabolomics analysis indicated that QS-related metabolites, such as ethanolamine and L-methionine, were down-regulated after treatment with DCA and MCA. Quantitative real-time polymerase chain reaction (qRT-PCR) demonstrated that DCA and MCA markedly suppressed the expression of two QS-related genes (cviI and cviR). In addition, DCA and MCA also inhibited biofilm formation and enhanced the susceptibility of biofilms to tobramycin, which was evidenced by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Our results indicated that DCA and MCA can serve as QS-based agent for controlling pathogens.Key Points • DCA and MCA inhibited QS and biofilm formation in C. violaceum.• The combination of DCA or MCA and tobramycin removed the preformed biofilm of C. violaceum. • DCA or MCA inhibited virulence factors and expressions of cviI and cviR of C. violaceum.• DCA or MCA are potential antibiotic accelerants for treating C. violaceum infection.
中文翻译:
两种天然肉桂酸衍生物在紫细菌中对群体感应的抑制作用和妥布霉素的促进作用。
紫色杆菌是一种自由生存的革兰氏阴性细菌,在热带和亚热带的土壤和水生环境中大量存在。它也是机会性人类病原体。在这里,两种肉桂酸衍生物,即4-二甲基氨基肉桂酸(DCA)和4-甲氧基肉桂酸(MCA),被鉴定为紫胶衣ATCC12472中潜在的群体感应(QS)和生物膜抑制剂。DCA(100μg/ mL)和MCA(200μg/ mL)均抑制N.癸酰-高丝氨酸内酯(C10-HSL)的水平并减少紫胶衣藻中某些毒力因子的产生,包括紫胶菌素,溶血素和几丁质酶。代谢组学分析表明,用DCA和MCA处理后,与QS相关的代谢产物(如乙醇胺和L-蛋氨酸)被下调。实时定量聚合酶链反应(qRT-PCR)表明DCA和MCA显着抑制了两个QS相关基因(cviI和cviR)的表达。此外,DCA和MCA还抑制生物膜的形成并增强生物膜对妥布霉素的敏感性,这通过扫描电子显微镜(SEM)和共聚焦激光扫描显微镜(CLSM)得以证明。我们的结果表明,DCA和MCA可以作为基于QS的病原体控制剂。要点•DCA和MCA抑制了紫胶衣藻的QS和生物膜形成。•DCA或MCA与妥布霉素的组合去除了预先形成的C生物膜。紫胶 •DCA或MCA抑制了毒力因子以及紫胶衣藻cviI和cviR的表达。•DCA或MCA是治疗紫胶衣藻感染的潜在抗生素促进剂。
更新日期:2020-04-04
中文翻译:
两种天然肉桂酸衍生物在紫细菌中对群体感应的抑制作用和妥布霉素的促进作用。
紫色杆菌是一种自由生存的革兰氏阴性细菌,在热带和亚热带的土壤和水生环境中大量存在。它也是机会性人类病原体。在这里,两种肉桂酸衍生物,即4-二甲基氨基肉桂酸(DCA)和4-甲氧基肉桂酸(MCA),被鉴定为紫胶衣ATCC12472中潜在的群体感应(QS)和生物膜抑制剂。DCA(100μg/ mL)和MCA(200μg/ mL)均抑制N.癸酰-高丝氨酸内酯(C10-HSL)的水平并减少紫胶衣藻中某些毒力因子的产生,包括紫胶菌素,溶血素和几丁质酶。代谢组学分析表明,用DCA和MCA处理后,与QS相关的代谢产物(如乙醇胺和L-蛋氨酸)被下调。实时定量聚合酶链反应(qRT-PCR)表明DCA和MCA显着抑制了两个QS相关基因(cviI和cviR)的表达。此外,DCA和MCA还抑制生物膜的形成并增强生物膜对妥布霉素的敏感性,这通过扫描电子显微镜(SEM)和共聚焦激光扫描显微镜(CLSM)得以证明。我们的结果表明,DCA和MCA可以作为基于QS的病原体控制剂。要点•DCA和MCA抑制了紫胶衣藻的QS和生物膜形成。•DCA或MCA与妥布霉素的组合去除了预先形成的C生物膜。紫胶 •DCA或MCA抑制了毒力因子以及紫胶衣藻cviI和cviR的表达。•DCA或MCA是治疗紫胶衣藻感染的潜在抗生素促进剂。