Staphylococcus aureus is a notorious foodborne pathogen since it has ability to produce variety of toxins including heat-stable enterotoxin, form biofilm, and acquire resistance to antibiotics. Biocontrol of foodborne pathogens by lytic bacteriophages garners increasing interest from both researchers and food industry. In the present study, 29 phages against S. aureus were successfully isolated from chicken, pork, and fish. Characterization of the isolates revealed that phage SA46-CTH2 belonging to Podoviridae family had a number of features suitable for food industry applications such as wide host range, short latent period, large burst size, high stress tolerance, and a genome free of virulence genes. Furthermore, phage SA46-CTH2 alone or in combination with nisin exhibited great efficacy in reducing planktonic and biofilm cells of S. aureus at various conditions tested. The combination of phage SA46-CTH2 and nisin was also found to be able to inhibit the regrowth of S. aureus at both 37 and 24 °C.Key points• A total of 29 S. aureus phages were successfully isolated from fish, pork, and chicken products. • Phage SA46-CTH2 was characterized by host range, morphology, and genome sequencing. • SA46-CTH2 significantly reduced both planktonic and biofilm cells of S. aureus. • Combination of SA46-CTH2 and nisin inhibited the regrowth of S. aureus.

中文翻译：

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单独或与乳链菌肽结合使用的噬菌体针对金黄色葡萄球菌的浮游生物膜和生物膜细胞的分离和应用。

金黄色葡萄球菌是一种臭名昭著的食源性病原体，因为它能够产生多种毒素，包括热稳定的肠毒素，形成生物膜并获得对抗生素的抗性。溶菌性噬菌体对食源性病原体的生物控制引起了研究人员和食品工业的越来越多的兴趣。在本研究中，成功​​地从鸡，猪肉和鱼中分离出了29种针对金黄色葡萄球菌的噬菌体。分离株的表征表明，属于猪痘病毒科的噬菌体SA46-CTH2具有许多适合食品工业应用的特征，例如宿主范围广，潜伏期短，爆发强度大，耐逆性高，基因组不含毒力基因。此外，噬菌体SA46-CTH2单独或与乳链菌肽结合在减少S的浮游和生物膜细胞方面显示出巨大的功效。在各种条件下测试金黄色葡萄球菌。还发现噬菌体SA46-CTH2和乳链菌肽的组合在37和24°C时均能抑制金黄色葡萄球菌的再生。要点•从鱼，猪肉，和鸡肉产品。•噬菌体SA46-CTH2的特点是宿主范围，形态和基因组测序。•SA46-CTH2显着减少了金黄色葡萄球菌的浮游和生物膜细胞。•SA46-CTH2和乳链菌肽的结合可抑制金黄色葡萄球菌的再生。和基因组测序。•SA46-CTH2显着减少了金黄色葡萄球菌的浮游和生物膜细胞。•SA46-CTH2和乳链菌肽的结合可抑制金黄色葡萄球菌的再生。和基因组测序。•SA46-CTH2显着减少了金黄色葡萄球菌的浮游和生物膜细胞。•SA46-CTH2和乳链菌肽的结合可抑制金黄色葡萄球菌的再生。