Candida albicans invasion is one of the most serious fungal infections in clinical history. In recent years, because of the widespread use of immunosuppressive drugs, chemotherapy drugs, glucocorticoids, and broad-spectrum antibiotics, serious drug resistance has been reported; therefore, a new type of antifungal drug needs to be developed. In this study, we found that Nerol (NEL) had strong antimicrobial activity and 0.77 μL/mL NEL was the minimum inhibitory concentration (MIC) effective against C. albicans. We determined the change of the growth curve of NEL for C. albicans, to identify the trend of NEL activity against C. albicans. Through the determination of the ergosterol content and glucose-induced extracellular fluid acidification of NEL on C. albicans, we found that NEL inhibits the growth of C. albicans by destroying cell membranes. This finding was also supported by the expression of SAP (secreted aspartyl proteinase) involved in cell membrane synthesis. Finally, demonstrations of phenotype investigation, colony-forming unit (CFU) counts, and PAS (periodic acid-Schiff) staining were conducted to prove that NEL had the ability to treated mouse oral C. albicans infection and vaginal C. albicans infection. This research may help us to investigate new antimicrobial agents for treating C. albicans infections. KEY POINTS: • NEL can inhibit the growth of C. albicans. • NEL destroys the cell membrane formation and permeability of C. albicans. • NEL can treat vulvovaginal candidiasis and oropharyngeal candidiasis in mice. • NEL could be used as a possible antifungal agent.

中文翻译：

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天然植物活性成分Nerol在体外和体内对白色念珠菌的活性。

白色念珠菌入侵是临床历史上最严重的真菌感染之一。近年来，由于免疫抑制药物，化学疗法药物，糖皮质激素和广谱抗生素的广泛使用，已报道了严重的耐药性。因此，需要开发一种新型的抗真菌药。在这项研究中，我们发现Nerol（NEL）具有很强的抗菌活性，0.77μL/ mL NEL是对白色念珠菌有效的最小抑菌浓度（MIC）。我们确定了白色念珠菌的NEL生长曲线的变化，以确定NEL对白色念珠菌的活性趋势。通过测定麦角固醇含量和葡萄糖诱导的白色念珠菌细胞内液的酸化，我们发现NEL通过破坏细胞膜抑制白色念珠菌的生长。参与细胞膜合成的SAP（分泌的天冬氨酰蛋白酶）的表达也支持了这一发现。最后，进行了表型研究，集落形成单位（CFU）计数和PAS（高碘酸席夫（Schiff）染色）的证明，证明NEL具有治疗小鼠口腔白色念珠菌感染和阴道白色念珠菌感染的能力。这项研究可能有助于我们研究用于治疗白色念珠菌感染的新型抗菌剂。要点：•NEL可以抑制白色念珠菌的生长。•NEL破坏白色念珠菌的细胞膜形成和通透性。•NEL可以治疗小鼠的阴道阴道念珠菌病和口咽念珠菌病。•NEL可用作可能的抗真菌剂。