Collagen and polysaccharide materials have great advantages for burn wound healing and skin regeneration. The purpose of this study was to develop a promising burn dressing. Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker. The mechanical properties and pore size of the hydrogel were optimized by response surface methodology. The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm. This study constructed a skin burn model and demonstrated that the hydrogel dressing could effectively prevent bacteria infection and confirmed that the hydrogel dressing was more beneficial for promoting burn wound healing than a commercial film (DUO DERM). Therefore, the HLC/HA/CCS hydrogel can be considered a promising burn wound dressing.

中文翻译：

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使用响应表面进行烧伤修复的人样胶原蛋白复合多糖水凝胶敷料的优化制备。

胶原蛋白和多糖材料对于烧伤伤口愈合和皮肤再生具有巨大优势。这项研究的目的是开发一种有前途的烧伤敷料。混合透明质酸（HA），羧化壳聚糖（CCS）和类人胶原（HLC）以模拟细胞外基质（ECM），并将谷氨酰胺转氨酶（TG）用作交联剂。通过响应表面方法优化了水凝胶的机械性能和孔径。结果表明，水凝胶的拉伸弹性模量为480.43±15.82kPa，拉伸应变为55.23±2.43％，孔径为90.43±5.57μm。这项研究构建了皮肤烧伤模型，并证明了水凝胶敷料可以有效防止细菌感染，并证实了水凝胶敷料比商业用膜（DUO DERM）更有利于促进烧伤创面愈合。因此，HLC / HA / CCS水凝胶可被视为有前途的烧伤创面敷料。