The health risks of exposure to plasticizers have received widespread attention, however, little is known about the effects of fast food packaging plasticizers on steroid hormone synthesis. In the present study, the types and migration of plasticizers in some commonly used fast-food packaging materials were detected by GC-MS, and the interference effects of these plasticizers and their metabolites on steroid hormone synthesis in the human body were evaluated by the H295R steroidogenesis assay. The GC-MS results showed that the main plasticizer compounds that migrated from fast food packaging into food were di(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) and bis(2-ethylhexyl) adipate (DEHA). Exposure to these chemicals (100-1000 μM) can significantly reduce the viability of H295R cells in a dose-response manner, and these plasticizers and their metabolites that migrated into oily foods at high temperatures (0.25-25 μM) could significantly increase the E2 level and reduce the T level in H295R cells. According to the qRT-PCR data, 0.25 to 25 μM mono(2-ethylhexyl) phthalate (MEHP) significantly upregulated the expression levels of 17β-HSD1 and CYP19A1, and downregulated those of CYP17A1, CYP11A1 and StAR. The Western blot results were consistent with those of qRT-PCR. In summary, these results indicated that even exposure to low concentrations (≤1 mg/l or 2.5 μM) of these chemicals and their metabolites can cause significant endocrine-disrupting effects.

中文翻译：

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快餐包装增塑剂及其代谢产物对H295R细胞中类固醇激素合成的影响。

暴露于增塑剂的健康风险已受到广泛关注，但是，快餐包装增塑剂对类固醇激素合成的影响知之甚少。在本研究中，通过GC-MS检测了一些常用快餐包装材料中增塑剂的类型和迁移，并通过H295R评估了这些增塑剂及其代谢产物对人体类固醇激素合成的干扰作用。类固醇生成测定。GC-MS结果表明，从快餐包装迁移到食品中的主要增塑剂化合物是邻苯二甲酸二（2-乙基己基）酯，邻苯二甲酸二丁酯（DBP）和己二酸双（2-乙基己基）酯（DEHA）。暴露于这些化学物质（100-1000μM）会以剂量反应方式显着降低H295R细胞的生存能力，并且这些增塑剂及其代谢物在高温（0.25-25μM）下迁移到油性食物中，可以显着提高H295R细胞的E2水平并降低T水平。根据qRT-PCR数据，0.25至25μM邻苯二甲酸单（2-乙基己基）酯（MEHP）显着上调17β-HSD1和CYP19A1的表达水平，并下调CYP17A1，CYP11A1和StAR的表达水平。Western印迹结果与qRT-PCR结果一致。总之，这些结果表明，即使暴露于低浓度（≤1mg / l或2.5μM）的这些化学物质及其代谢物也会引起明显的内分泌干扰作用。25至25μM邻苯二甲酸单（2-乙基己基）酯（MEHP）显着上调17β-HSD1和CYP19A1的表达水平，并下调CYP17A1，CYP11A1和StAR的表达水平。Western印迹结果与qRT-PCR结果一致。总之，这些结果表明，即使暴露于低浓度（≤1mg / l或2.5μM）的这些化学物质及其代谢物也会引起明显的内分泌干扰作用。25至25μM邻苯二甲酸单（2-乙基己基）酯（MEHP）显着上调17β-HSD1和CYP19A1的表达水平，并下调CYP17A1，CYP11A1和StAR的表达水平。Western印迹结果与qRT-PCR结果一致。总之，这些结果表明，即使暴露于低浓度（≤1mg / l或2.5μM）的这些化学物质及其代谢物也会引起明显的内分泌干扰作用。