BACKGROUND The present work aimed to detect the toxicological effects of green synthesized silver nanoparticles (Ag-NPs) by using Moringa Oleifera leaves extract on hematological and biochemical parameters of Oreochromis niloticus. METHODS Adult fish were exposed to two sublethal concentrations (1.95 and 3.9 ppm) of Ag-NPs against sodium selenite (0.1 ppm) and biosynthesized selenium nanoparticles (Se-NPs); 0.1 ppm; protection role for 2 and 4 weeks. Hematological parameters; erythrocyte count (RBCs), hemoglobin content (Hb), haematocrit value (Hct), mean corpuscular volume (MCV), Mean Corpuscular Hemoglobin Concentration (MCHC), leucocytes (WBCs), with differential count Micronucleus (MN) and alerted cells and biochemical parameters; aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) enzyme activities, serum protein (total protein, albumin and globulin) concentration, urea, creatinine, glucose, cholesterol (Cho) and triglyceride (Tg) were detected. RESULTS The present investigation showed that Ag-NPs in different doses led to a significant reduction (p < 0.05) in RBCs, Hb, Hct, MCV, WBCs, LYM and serum proteins concentration. However, MCHC, MN, alerted cells, NEUT, AST, ALT, ALP enzyme activities, urea, creatinine, glucose, Cho and Tg showed a significant increases (p < 0.05) when compared with control group. Sodium selenite (Se) and biosynthesized selenium nanoparticles (Se-NPs) play an optimistic role in detoxification of Ag-NPs toxicity. CONCLUSION The results suggest the negative impact of Ag-NPs on hematology and biochemical parameters of fish. Moreover, Se-NPs showed a full improvement of hematological and biochemical parameters more than that of sodium selenite in elimination of Ag-NPs toxicity.

中文翻译：

---

绿色合成的银纳米颗粒的毒理学影响和不同硒类型对尼罗罗非鱼的保护作用：血液学和生化反应。

背景技术目前的工作旨在通过使用辣木叶提取物对尼罗罗非鱼的血液学和生化指标检测绿色合成的银纳米颗粒（Ag-NPs）的毒理作用。方法成鱼鱼暴露于两种亚致死浓度（1.95和3.9 ppm）的银-NPs对亚硒酸钠（0.1 ppm）和生物合成的硒纳米颗粒（Se-NPs）；0.1 ppm；保护作用持续2周和4周。血液学参数 红细胞计数（RBC），血红蛋白含量（Hb），血细胞比容值（Hct），平均红细胞体积（MCV），平均红细胞血红蛋白浓度（MCHC），白细胞（WBCs），微分计数（MN）以及警报细胞和生化指标参数; 天冬氨酸转氨酶（AST），丙氨酸转氨酶（ALT），碱性磷酸酶（ALP）的酶活性，检测血清蛋白（总蛋白，白蛋白和球蛋白）浓度，尿素，肌酐，葡萄糖，胆固醇（Cho）和甘油三酸酯（Tg）。结果本研究表明，不同剂量的Ag-NPs导致RBC，Hb，Hct，MCV，WBC，LYM和血清蛋白浓度显着降低（p <0.05）。然而，与对照组相比，MCHC，MN，警觉细胞，NEUT，AST，ALT，ALP酶活性，尿素，肌酐，葡萄糖，Cho和Tg显着增加（p <0.05）。亚硒酸钠（Se）和生物合成的硒纳米颗粒（Se-NPs）在解毒Ag-NPs的毒性中起着乐观的作用。结论结果表明Ag-NPs对鱼类血液学和生化参数的负面影响。此外，