A novel electrochemical aptasensor modified with highly porous gold and aptamer was prepared for the determination of acetamiprid in fruits and vegetables. Highly porous gold was synthesized by electroreduction at -4 V in an electrolyte containing 2.5 mol/L NH4Cl and 10 mmol/L HAuCl4. Acetamiprid-binding aptamer was immobilized on highly porous gold by self-assembly. Acetamiprid could be captured by aptamer on the sensing interface, resulting in an increment of electron transfer resistance. Thanks to the large specific surface area of highly porous gold and the high affinity of aptamer, the aptasensor exhibited a highly sensitive impedance response for acetamiprid. Under optimal condition, the aptasensor displayed a linear response for acetamiprid in the concentration range of 0.5-300 nmol/L, and the detection limit was 0.34 nmol/L. Furthermore, the aptasensor showed high selectivity, good reproducibility and stability. Finally, the aptasensor was applied for the determination of acetamiprid in fruits and vegetables with satisfactory results.

中文翻译：

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基于高度多孔的金的阻抗适体传感器，用于水果和蔬菜中对乙酰氨基酚的灵敏检测。

制备了一种用高孔金和适体修饰的新型电化学适体传感器，用于测定水果和蔬菜中的扑热息痛。通过在含有2.5 mol / L NH4Cl和10 mmol / L HAuCl4的电解质中-4 V进行电还原合成高度多孔的金。通过自组装将对乙酰氨基酚结合的适体固定在高度多孔的金上。扑热息痛可被适体在传感界面上捕获，从而导致电子转移电阻增加。由于高度多孔的金的大的比表面积和适体的高亲和力，适体传感器对乙酰胺显示出高度敏感的阻抗响应。在最佳条件下，适体传感器在0.5-300 nmol / L的浓度范围内对乙酰胺具有线性响应，检出限为0.34 nmol / L。此外，适体传感器显示出高选择性，良好的重现性和稳定性。最后，将适体传感器用于水果和蔬菜中扑热息痛的测定，结果令人满意。