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Fluorescent aptasensors for parallel analysis of biomolecules based on interlocked DNA catenane nanomachines
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.aca.2020.04.004
Hong Liao 1 , Ting Huang 2 , Lianzhe Hu 1 , Min Wang 2
Affiliation  

Stimuli-responsive DNA catenane nanomachines have received considerable interest in the area of DNA nanotechnology. However, the sensing and bio-sensing applications of DNA catenane nanomachines are rarely explored. Herein, the biological small molecule and protein responsive DNA catenane nanomachines were designed by utilizing the specific aptamer/target interaction. In the presence of the target, the blocked catalytically inactive DNA catenane can be activated, while generating the metal ion-dependent DNAzyme as the signal output unit. The method allows the fluorescent detection of ATP and lysozyme with a detection limit of 20 nM and 200 pM, respectively. More importantly, through the use of different fluorophores as labels, the parallel analysis of ATP and lysozyme in the mixed solution was demonstrated based on two interlocked DNA catenanes. Compared with linear scaffold, the interlocked DNA rings showed enhanced stability against enzyme degradation and easily realized intramolecular reconfiguration. Due to its inherent advantages, the interlocked DNA catenane holds great promise in the field of DNA based sensors and nanodevices.

中文翻译:

基于互锁 DNA 链纳米机器的用于平行分析生物分子的荧光适体传感器

刺激响应性 DNA 链状纳米机器在 DNA 纳米技术领域引起了相当大的兴趣。然而,DNA 链纳米机器的传感和生物传感应用很少被探索。在此,利用特定的适体/靶标相互作用设计了生物小分子和蛋白质响应性 DNA 链纳米机器。在靶标存在的情况下,被阻断的催化失活的 DNA 链环可以被激活,同时产生金属离子依赖性 DNAzyme 作为信号输出单元。该方法允许对 ATP 和溶菌酶进行荧光检测,检测限分别为 20 nM 和 200 pM。更重要的是,通过使用不同的荧光团作为标记,混合溶液中的 ATP 和溶菌酶的平行分析是基于两个互锁的 DNA 链。与线性支架相比,互锁的 DNA 环显示出增强的抗酶降解稳定性,并且易于实现分子内重构。由于其固有的优势,互锁的 DNA 链在基于 DNA 的传感器和纳米设备领域具有广阔的前景。
更新日期:2020-06-01
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