The shelf-life of human platelets preserved in vitro for therapeutic transfusion is limited because of bacterial contamination and platelet storage lesion (PSL). The PSL is the predominant factor and limiting unfavorable interactions between the platelets and the non-biocompatible storage bag surfaces is the key to alleviate PSL. Here we describe a surface modification method for biocompatible platelet storage bags that dramatically extends platelet shelf-life beyond the current US Food and Drug Administration (FDA) standards of 5 days. The surface coating of the bags can be achieved through a simple yet effective dip-coating and light-irradiation method using a biocompatible polymer. The biocompatible polymers with tunable functional groups can be routinely fabricated at any scale and impart super-hydrophilicity and non-fouling capability on commercial hydrophobic platelet storage bags. As critical parameters reflecting the platelets quality, the activation level and binding affinity with von Willebrand factor (VWF) of the platelets stored in the biocompatible platelet bags at 8 days are comparable with those in the commercial bags at 5 days. This technique also demonstrates promise for a wide range of medical and engineering applications requiring biocompatible surfaces. STATEMENT OF SIGNIFICANCE: Current standard platelet preservation techniques agitate platelets at room temperature (20-24 °C) inside a hydrophobic (e.g., polyvinyl chloride (PVC)) storage bag, thereby allowing preservation of platelets only for 5 days. A key factor leading to quality loss is the unfavorable interaction between the platelets and the non-biocompatible storage bag surfaces. Here, a surface modification method for biocompatible platelet storage bags has been created to dramatically extend platelet shelf-life beyond the current FDA standards of 5 days. The surface coating of the bags can be achieved via a simple yet effective dip-coating and light-irradiation method using a carboxybetaine polymer. This technique is also applicable to many other applications requiring biocompatible surfaces.

中文翻译：

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两性离子羧基甜菜碱聚合物可延长人血小板的保存期限。

由于细菌污染和血小板存储病变（PSL），体外保存用于治疗性输血的人类血小板的货架期受到限制。PSL是主要因素，限制血小板与非生物相容性储物袋表面之间不利的相互作用是缓解PSL的关键。在这里，我们描述了一种用于生物相容性血小板存储袋的表面修饰方法，该方法可大大延长血小板的保存期限，使之超过当前的美国食品药品监督管理局（FDA）的5天标准。可以通过使用生物相容性聚合物的简单而有效的浸涂和光照射方法来实现袋子的表面涂层。具有可调官能团的生物相容性聚合物可按常规规模制造，并在商用疏水性血小板存储袋上具有超亲水性和不结垢的能力。作为反映血小板质量的关键参数，在第8天储存在生物相容性血小板袋中的血小板的活化水平和与von Willebrand因子（VWF）的结合亲和力与在商用袋中第5天的血小板相当。该技术还证明了需要生物相容性表面的广泛医学和工程应用的前景。意义声明：当前的标准血小板保存技术是在室温（20-24°C）的疏水性（例如聚氯乙烯（PVC））存储袋内搅动血小板，从而仅将血小板保存5天。导致质量下降的关键因素是血小板与非生物相容性存储袋表面之间的不利相互作用。在这里，已经创建了一种用于生物相容性血小板存储袋的表面修饰方法，以大大延长血小板的货架期，使其超过目前的FDA标准（5天）。可以通过使用羧基甜菜碱聚合物的简单而有效的浸涂和光照射方法来实现袋的表面涂覆。该技术还适用于需要生物相容性表面的许多其他应用。已经开发出一种用于生物相容性血小板存储袋的表面修饰方法，以大大延长血小板的保质期，使其超过目前的FDA标准（5天）。可以通过使用羧基甜菜碱聚合物的简单而有效的浸涂和光照射方法来实现袋的表面涂覆。该技术还适用于需要生物相容性表面的许多其他应用。已经开发出一种用于生物相容性血小板存储袋的表面修饰方法，以大大延长血小板的保质期，使其超过目前的FDA标准（5天）。可以通过使用羧基甜菜碱聚合物的简单而有效的浸涂和光照射方法来实现袋的表面涂覆。该技术还适用于需要生物相容性表面的许多其他应用。