Municipal effluents have the potential to disrupt the endocrine system involved in reproduction in aquatic organisms. The purpose of this study was to develop a novel a quantitative polymerase chain reaction (qPCR) array for the freshwater mussel Elliptio complanata to compare the toxic properties of municipal effluents and rainfall overflows. Mussels were caged for 3 months at upstream and downstream sites of a municipal effluent discharge point and 2 rainfall overflow sites in the Saint-Lawrence River. The data revealed that exposure to municipal effluents and overflow sites lead to increased expression of vitellogenin (VTG) in male mussels, altered sexual differentiation in females, gene expression involved in oxidative stress (superoxide dismutase, glutathione S-transferase) and DNA damage (chromosome mismatch and repair of covalently-bound DNA adducts). Mussels at the downstream site accumulated also large amounts of heterotrophic bacteria but not at the overflow sites. However, mussels at the overflow sites had decreased expression in ABC transporter gene expression. Scaling analysis revealed that the following gene expression were related to effects at higher level of biological organization such as total RNA levels, gonad mass and gonad somatic index: DNA repair of covalent DNA adducts, mistmach chromosomal recombination during meiosis and undifferentiated cells in gonads. In conclusion, exposure to rainfall overflow sites have similar effects to municipal effluents based on VTG and oxidative stress responses at the transcriptomic levels only but the effects at the DNA integrity and sexual differentiation were involved in adverse outcome pathways of urban pollution in Elliptio complanata mussels.

中文翻译：

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使用新型qPCR阵列方法比较Elliptio complanata中降雨溢流和市政污水羽流的相对毒性。

市政污水有可能破坏与水生生物繁殖有关的内分泌系统。这项研究的目的是为淡水贻贝Elliptio complanata开发一种新型的定量聚合酶链反应（qPCR）阵列，以比较市政污水和降雨溢流的毒性。贻贝在市政污水排放点的上游和下游站点以及圣劳伦斯河的2个降雨溢流站点被关押了3个月。数据显示，暴露于市政污水和溢流部位会导致雄性贻贝中卵黄蛋白原（VTG）的表达增加，雌性的性分化改变，氧化应激（超氧化物歧化酶，谷胱甘肽S-转移酶）和DNA损伤（染色体错配和共价结合的DNA加合物的修复）。贻贝在下游部位也积累了大量异养细菌，但在溢流部位却没有。但是，在溢流部位的贻贝在ABC转运蛋白基因表达中的表达下降。缩放分析显示以下基因表达与更高水平的生物组织作用有关，例如总RNA水平，性腺质量和性腺体细胞指数：共价DNA加合物的DNA修复，减数分裂过程中的雾状染色体重组以及性腺中未分化的细胞。结论，