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Exploring the roles of fecundity-related long non-coding RNAs and mRNAs in the adrenal glands of small-tailed Han Sheep.
BMC Genetics ( IF 2.9 ) Pub Date : 2020-04-06 , DOI: 10.1186/s12863-020-00850-6
Qing Xia 1 , Qiuling Li 2 , Shangquan Gan 3 , Xiaofei Guo 4 , Xiaosheng Zhang 4 , Jinlong Zhang 4 , Mingxing Chu 1
Affiliation  

BACKGROUND Long non-coding RNAs (lncRNAs) can play important roles in uterine and ovarian functions. However, little researches have been done on the role of lncRNAs in the adrenal gland of sheep. Herein, RNA sequencing was used to compare and analyze gene expressions in adrenal tissues between follicular phases and luteal phases in FecBBB (MM) and FecB++ (WW) sheep, respectively, and differentially expressed lncRNAs and genes associated with reproduction were identified. RESULTS In MM sheep, 38 lncRNAs and 545 mRNAs were differentially expressed in the adrenal gland between the luteal and follicular phases; In WW sheep, 513 differentially expressed lncRNAs and 2481 mRNAs were identified. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses indicated that differentially expressed lncRNAs and their target genes are mainly involved in the circadian rhythm, the mitogen activated protein kinase, thyroid, ovarian steroidogenesis and transforming growth factor beta signaling pathways. Differentially expressed lncRNAs can regulate reproduction by modulating genes involved in these signaling pathways and biological processes. Specifically, XLOC_254761, XLOC_357966, 105,614,839 and XLOC_212877 targeting CREB1, PER3, SMAD1 and TGFBR2, respectively, appear to play key regulatory roles. CONCLUSION These results broaden our understanding of lncRNAs in adrenal gland of sheep and provide new insights into the molecular mechanisms underlying sheep reproduction.

中文翻译:

探讨小尾寒羊肾上腺中与繁殖力相关的长非编码RNA和mRNA的作用。

背景长链非编码RNA(lncRNA)在子宫和卵巢功能中发挥重要作用。然而,关于lncRNA在绵羊肾上腺中的作用的研究还很少。本文通过RNA测序分别比较分析FecBBB(MM)和FecB++(WW)羊卵泡期和黄体期肾上腺组织中的基因表达,鉴定差异表达的lncRNA和与繁殖相关的基因。结果 在 MM 羊中,黄体期和卵泡期肾上腺中 38 个 lncRNA 和 545 个 mRNA 有差异表达;在WW羊中,鉴定出513个差异表达的lncRNA和2481个mRNA。Gene Ontology和京都基因与基因组百科全书富集分析表明,差异表达的lncRNA及其靶基因主要涉及昼夜节律、丝裂原激活蛋白激酶、甲状腺、卵巢类固醇生成和转化生长因子β信号通路。差异表达的lncRNA可以通过调节参与这些信号通路和生物过程的基因来调节繁殖。具体而言,分别针对CREB1、PER3、SMAD1和TGFBR2的XLOC_254761、XLOC_357966、105,614,839和XLOC_212877似乎发挥关键的调节作用。结论这些结果拓宽了我们对绵羊肾上腺lncRNA的理解,并为绵羊繁殖的分子机制提供了新的见解。
更新日期:2020-04-22
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