Protein turnover, the net result of protein synthesis and degradation, enables cells to remodel their proteomes in response to internal and external cues. Previously, we analyzed protein turnover rates in cultured brain cells under basal neuronal activity and found that protein turnover is influenced by subcellular localization, protein function, complex association, cell type of origin, and by the cellular environment (Dörrbaum et al., 2018). Here, we advanced our experimental approach to quantify changes in protein synthesis and degradation, as well as the resulting changes in protein turnover or abundance in rat primary hippocampal cultures during homeostatic scaling. Our data demonstrate that a large fraction of the neuronal proteome shows changes in protein synthesis and/or degradation during homeostatic up- and down-scaling. More than half of the quantified synaptic proteins were regulated, including pre- as well as postsynaptic proteins with diverse molecular functions.

中文翻译：

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在培养的神经元稳态缩放过程中的蛋白质组动力学。

蛋白质更新是蛋白质合成和降解的最终结果，它使细胞能够根据内部和外部提示重塑其蛋白质组。以前，我们分析了基础神经元活动下培养的脑细胞中的蛋白质周转率，发现蛋白质周转受亚细胞定位，蛋白质功能，复杂缔合，起源细胞类型以及细胞环境的影响（Dörrbaumet al。，2018）。 。在这里，我们改进了我们的实验方法，以量化稳态稳态缩放过程中大鼠原代海马培养物中蛋白质合成和降解的变化以及蛋白质周转率或丰度的变化。我们的数据表明，神经元蛋白质组的很大一部分在稳态放大和缩小过程中显示出蛋白质合成和/或降解的变化。