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Nano-vesicular formulation of propolis and cytotoxic effects in a 3D spheroid model of lung cancer
Journal of the Science of Food and Agriculture ( IF 4.1 ) Pub Date : 2020-04-18 , DOI: 10.1002/jsfa.10400
Esra Ilhan-Ayisigi 1, 2 , Fulden Ulucan 3 , Ecem Saygili 1 , Pelin Saglam-Metiner 1 , Sultan Gulce-Iz 1, 3, 4 , Ozlem Yesil-Celiktas 1, 3
Affiliation  

BACKGROUND Propolis exhibits therapeutic properties due to the presence of phenolic acids, esters and flavonoids. The scope of this study was to develop a nano-vesicular formulation and establish a 3-Dimensional (3D) spheroid model, in which lung cancer is recapitulated. RESULTS Niosome vesicles doped with galangin rich propolis extract were synthesized by ether injection method using a cholesterol:surfactant mass ratio of 1:3 at 40 °C for 1 h. Formulated niosomes were administered to 3D lung cancer spheroid model and the cytotoxicity was compared with that of 2-Dimensional (2D) setting. Galangin content was determined as 86 μg/mg propolis extract by UPLC. The particle size of loaded niosome was 151 ± 2.84 nm with a polydispersity index (PDI) of about 0.232 and an encapsulation efficiency of 70% was achieved. CONCLUSION The decrease in cell viability and scattering in the 3D spheroids of A549 lung cancer cells treated with propolis loaded niosomes were notable indicating a profound cytotoxic effect as well suggesting utilization as an effective nano-vesicle. This article is protected by copyright. All rights reserved.

中文翻译:

蜂胶的纳米囊泡配方和细胞毒性作用在肺癌 3D 球体模型中

背景技术蜂胶由于酚酸、酯和类黄酮的存在而表现出治疗特性。本研究的范围是开发纳米囊泡制剂并建立 3 维 (3D) 球体模型,其中概括了肺癌。结果 通过醚注射法合成了富含高良姜素的蜂胶提取物的 Niosome 囊泡,胆固醇:表面活性剂的质量比为 1:3,在 40 °C 下 1 小时。将配制的 niosome 施用于 3D 肺癌球体模型,并将细胞毒性与二维 (2D) 设置进行比较。通过 UPLC 测定高良姜素含量为 86 μg/mg 蜂胶提取物。负载的 niosome 的粒径为 151 ± 2.84 nm,多分散指数 (PDI) 约为 0.232,封装效率达到 70%。结论 用负载蜂胶的 niosome 处理的 A549 肺癌细胞的 3D 球体中细胞活力和散射的降低是显着的,表明具有深刻的细胞毒性作用,并表明可用作有效的纳米囊泡。本文受版权保护。版权所有。
更新日期:2020-04-18
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