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Melanization induced by Heliothis virescens ascovirus 3h promotes viral replication
Insect Science ( IF 4 ) Pub Date : 2020-06-25 , DOI: 10.1111/1744-7917.12786
Zi-Qi Li 1, 2 , Xiao-Hui Song 1, 2 , Min Wang 1, 2 , Shu Wang 1, 2 , Guo-Hua Huang 1, 2
Affiliation  

Melanization is an important innate immune defense mechanism of insects, which can kill invading pathogens. Most pathogens, for their survival and reproduction, inhibit the melanization of the host. Interestingly, our results suggested that after infection with Heliothis virescens ascovirus 3h (HvAV-3h), the speed of melanization in infected Spodoptera exigua larval hemolymph was accelerated and that the phenoloxidase (PO) activity of hemolymph in larvae infected with HvAV-3h increased significantly (1.20-fold at 96 hpi, 1.52-fold at 120 hpi, 1.23-fold at 144 hpi, 1.12-fold at 168 hpi). The transcription level of the gene encoding S. exigua prophenoloxidase-1 (SePPO-1 gene) was upregulated dramatically in the fat body during the middle stage of infection. In addition, when melanization was inhibited or promoted, the replication of HvAV-3h was inhibited or promoted, respectively. In conclusion, infection with HvAV-3h can markedly induce melanization in the middle stage of infection, and melanization is helpful for HvAV-3h viral replication. This article is protected by copyright. All rights reserved.

中文翻译:

Heliothis virescens ascovirus 3h 诱导的黑色化促进病毒复制

黑色素化是昆虫重要的先天免疫防御机制,可以杀死入侵的病原体。大多数病原体,为了它们的生存和繁殖,会抑制宿主的黑化。有趣的是,我们的结果表明,感染黑夜蛾子囊病毒 3h(HvAV-3h)后,感染甜菜夜蛾幼虫血淋巴的黑色化速度加快,感染 HvAV-3h 幼虫血淋巴的酚氧化酶(PO)活性显着增加(96 hpi 时 1.20 倍,120 hpi 时 1.52 倍,144 hpi 时 1.23 倍,168 hpi 时 1.12 倍)。在感染中期,编码甜菜碱酚氧化酶原酶-1 基因(SePPO-1 基因)的转录水平在脂肪体内显着上调。此外,当黑色化受到抑制或促进时,HvAV-3h 的复制分别被抑制或促进。综上所述,感染HvAV-3h可显着诱导感染中期黑化,黑化有助于HvAV-3h病毒复制。本文受版权保护。版权所有。
更新日期:2020-06-25
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