We succeeded in the electrical polarization of β‐tricalcium phosphate (β‐TCP) granules and performed an unprecedented attempt to implant them into maxillary bone defects in canines to confirm their ability to facilitate new bone formation. Two holes were drilled into each maxilla half of a canine and filled with electrically polarized and nonpolarized β‐TCP granules (grouping assignment was decided randomly). The implanted specimens were dissected en bloc and used for microcomputed tomography (μCT) observations and histological analyses 4 and 8 weeks after the operation. New bone ingrowth in the bone hole progressed over time from the superficial layer of the cortex toward the inner cancellous bone. The percentage area of new bone in the bone hole, as measured by μCT in the sagittal plane, was significantly larger after 4 and 8 weeks, and that measured by H&E‐stained specimens in the transverse plane after 4 weeks was significantly larger in the polarized group than in the nonpolarized group. In addition to the structural stability and chemical characteristics of the β‐TCP granules, electrical stimulation bears influence not indirectly but directly on osteogenic and vessel cells, which might work cooperatively for the early initiation of the bone formation process.

中文翻译：

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通过电极化的 β-磷酸三钙移植物增强犬上颌骨的新骨形成。

我们成功地实现了 β-磷酸三钙 (β-TCP) 颗粒的电极化，并进行了前所未有的尝试，将它们植入犬的上颌骨缺损中，以确认它们促进新骨形成的能力。在犬的每个上颌骨上钻两个孔，并填充电极化和非极化的 β-TCP 颗粒（分组分配随机决定）。在手术后 4 周和 8 周，植入的标本被整体解剖并用于显微计算机断层扫描 (μCT) 观察和组织学分析。随着时间的推移，骨孔中的新骨向内生长，从皮质的表层向内松质骨发展。4 周和 8 周后，通过矢状面 μCT 测量的骨孔中新骨的百分比面积显着增大，4 周后横切面 H&E 染色标本测得的偏光组显着大于非偏光组。除了 β-TCP 颗粒的结构稳定性和化学特性外，电刺激对成骨细胞和血管细胞的影响不是间接而是直接的，这可能会协同作用以促进骨形成过程的早期启动。