ABSTRACT The 2019 new coronavirus epidemic potentially induced by wild animals has drawn tremendous attention. Wild animal meat contamination and adulteration have become increasingly serious, particularly for highly cooked wild animal meats that are difficult to be detected. In this study, a highly specific polyclonal antibody targeting the cooked rat proteins was developed. The corresponding sandwich ELISA (swELISA) was developed and found highly sensitive and specific for cooked rat meat, while there are no cross-reactions to the cooked chicken, pork and beef meats. The limit of detection (LOD) is determined to be as low as 0.01 ug/L based OD values. The coefficient variation (CV) is 5% and 8% for intra and inter assays, respectively. The recovery efficiencies are between 90% and 110%. The sandwich ELISA can detect both raw and cooked rat meat and is also suitable for Swab test of rat contamination. The results indicated a highly reliable and robust ELISA-based assay for cooked rat meat identification and contamination.

中文翻译：

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使用针对大鼠耐热蛋白的高度特异性抗体通过 ELISA 对煮熟的野生大鼠肉进行免疫测定

摘要：2019年新型冠状病毒疫情可能由野生动物引起，引起了极大关注。野生动物肉的污染和掺假问题日益严重，特别是对于难以检测的高度煮熟的野生动物肉。在这项研究中，开发了一种针对煮熟的大鼠蛋白质的高度特异性多克隆抗体。开发了相应的夹心ELISA（swELISA），发现对煮熟的大鼠肉高度敏感和特异，而对煮熟的鸡肉、猪肉和牛肉没有交叉反应。检测限 (LOD) 被确定为低至基于 0.01 ug/L 的 OD 值。内部和内部测定的系数变异 (CV) 分别为 5% 和 8%。回收效率在 90% 到 110% 之间。夹心ELISA可以检测生的和熟的大鼠肉，也适用于大鼠污染的拭子测试。结果表明，基于 ELISA 的高度可靠且稳健的检测方法可用于熟大鼠肉的识别和污染。