ABSTRACT Polysaccharides have various bioactivities including anti-tumour, antioxidant and immunoregulation, which are correlated with their structural characteristics. Here, four polysaccharides from Glycyrrhiza uralensis (GUPS), Pleurotus ferulae (PFPS), Triticum aestivum (TAPS) and Oryza sativa (OSPS) were purified to compare their structural characteristics and immunoregulatory activities. The data showed that TAPS, OSPS, GUPS and PFPS contained different monosaccharide components and had distinctly structural characteristics. All the four polysaccharides promoted the maturation of dendritic cells (DCs) through TLR4 with different activities. GUPS and PFPS also reduced the phagocytosis of DCs but not for TAPS and OSPS. Importantly, GUPS and PFPS enhanced the Th1 skewing immune response in mice without inflammation. TAPS and OSPS only showed weak immunostimulatory activity in vivo. The immunostimulatory activities of the four polysaccharides were ranked as PFPS = GUPS > TAPS > OSPS.

中文翻译：

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四种天然植物多糖结构特征及免疫调节活性比较

摘要 多糖具有多种生物活性，包括抗肿瘤、抗氧化和免疫调节，这与其结构特征相关。在这里，来自甘草 (GUPS)、阿魏 (PFP​​S)、小麦 (TAPS) 和水稻 (OSPS) 的四种多糖被纯化，以比较它们的结构特征和免疫调节活性。数据表明，TAPS、OSPS、GUPS和PFPS含有不同的单糖成分，具有明显的结构特征。四种多糖均通过具有不同活性的 TLR4 促进树突状细胞 (DC) 的成熟。GUPS 和 PFPS 也减少了 DCs 的吞噬作用，但对 TAPS 和 OSPS 没有。重要的是，GUPS 和 PFPS 增强了无炎症小鼠的 Th1 倾斜免疫反应。TAPS 和 OSPS 在体内仅表现出微弱的免疫刺激活性。四种多糖的免疫刺激活性排列为 PFPS = GUPS > TAPS > OSPS。