Summary

Transcription and DNA supercoiling are involved in a complex, dynamical and nonlinear coupling that results from the basal interaction between DNA and RNA Polymerase. We present the first software to simulate this coupling, applicable to a wide range of bacterial organisms. TwisTranscripT allows quantifying its contribution in global transcriptional regulation, and provides a mechanistic basis for the widely observed, evolutionarily conserved, and currently unexplained co-regulation of adjacent operons that might play an important role in genome evolution.

Availability and implementation

TwisTranscripT is freely available at https://github.com/sammeyer2017/TwisTranscripT. It is implemented in Python3 and supported on MacOS X, Linux and Windows.

中文翻译：

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TwisTranscripT：转录-超螺旋偶联的随机模拟。

概要

转录和DNA超螺旋涉及复杂的，动态的和非线性的偶联，该偶联是DNA和RNA聚合酶之间基础相互作用的结果。我们提供了第一个模拟这种耦合的软件，适用于多种细菌。TwisTranscripT可以量化其在全球转录调控中的作用，并为广泛观察到的，在进化上保守的和目前无法解释的对邻近操纵子的共调控提供机制基础，这些调控可能在基因组进化中发挥重要作用。

可用性和实施

TwisTranscripT可从https://github.com/sammeyer2017/TwisTranscripT免费获得。它在Python3中实现，并在MacOS X，Linux和Windows上受支持。