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β-Catenin and FGFR2 regulate postnatal rosette-based adrenocortical morphogenesis.
Nature Communications ( IF 16.6 ) Pub Date : 2020-04-03 , DOI: 10.1038/s41467-020-15332-7
Sining Leng 1, 2 , Emanuele Pignatti 1, 3 , Radhika S Khetani 4 , Manasvi S Shah 1, 3 , Simiao Xu 1, 3 , Ji Miao 1, 3 , Makoto M Taketo 5 , Felix Beuschlein 6, 7 , Paula Q Barrett 8 , Diana L Carlone 1, 3, 9 , David T Breault 1, 3, 9
Affiliation  

Rosettes are widely used in epithelial morphogenesis during embryonic development and organogenesis. However, their role in postnatal development and adult tissue maintenance remains largely unknown. Here, we show zona glomerulosa cells in the adult adrenal cortex organize into rosettes through adherens junction-mediated constriction, and that rosette formation underlies the maturation of adrenal glomerular structure postnatally. Using genetic mouse models, we show loss of β-catenin results in disrupted adherens junctions, reduced rosette number, and dysmorphic glomeruli, whereas β-catenin stabilization leads to increased adherens junction abundance, more rosettes, and glomerular expansion. Furthermore, we uncover numerous known regulators of epithelial morphogenesis enriched in β-catenin-stabilized adrenals. Among these genes, we show Fgfr2 is required for adrenal rosette formation by regulating adherens junction abundance and aggregation. Together, our data provide an example of rosette-mediated postnatal tissue morphogenesis and a framework for studying the role of rosettes in adult zona glomerulosa tissue maintenance and function.

中文翻译:

β-Catenin和FGFR2调节出生后基于玫瑰花结的肾上腺皮质形态发生。

莲座丛广泛用于胚胎发育和器官发生期间的上皮形态发生。但是,它们在产后发育和成年组织维持中的作用仍然未知。在这里,我们显示成年肾上腺皮质中的肾小球肾小球细胞通过粘附连接介导的收缩而组织成玫瑰花结,并且玫瑰花结的形成是出生后肾上腺肾小球结构成熟的基础。使用遗传小鼠模型,我们显示β-catenin的丧失导致粘附连接的破坏,花环数量减少和肾小球畸形,而β-catenin的稳定化导致粘附连接的丰度增加,更多的玫瑰花结和肾小球扩张。此外,我们发现了丰富的β-连环蛋白稳定的肾上腺丰富的上皮形态发生的已知调节器。在这些基因中,我们显示Fgfr2是通过调节粘附连接结的丰度和聚集来形成肾上腺玫瑰花所必需的。在一起,我们的数据提供了玫瑰花结节介导的产后组织形态发生的例子,以及研究玫瑰花结节在成年带状肾小球组织维持和功能中的作用的框架。
更新日期:2020-04-24
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