Microsatellite markers are a useful genetic tool to answer ecological and conservation genetics questions. Microsatellite primers were developed and characterized to evaluate forest fragmentation effects on genetic structure, diversity and gene flow patterns in the dioecious tropical tree Astroniumgraveolens (Anacardiaceae). Using genomic library enrichment, sixteen microsatellite loci were developed for A.graveolens. Polymorphism was evaluated in 80 individuals from four localities in Mexico. The overall number of alleles detected was 63, average alleles per locus 3.9 with a range from one to 11 per locus. Cross amplification trails on related species in the Anacardiaceae family: Spondiaspurpurea and Amphipterygiumadstringens, achieved successful amplification for all primers. Microsatellite markers described here are the first to be characterized for A.graveolens. These genetic markers will be a useful tool to assess the genetic consequences of habitat fragmentation and selective logging on A.graveolens populations. Cross amplification success on S.purpureaandA.adstringens suggest that they may be used for population genetic studies in other species within the family.

中文翻译：

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天文学家（Anacardiaceae）微卫星基因座的分离和鉴定以及相关物种的交叉扩增。

微卫星标记是回答生态学和保护遗传学问题的有用的遗传工具。开发了微卫星引物，并对其进行了表征，以评估森林片段化对雌雄异株热带天文学家天牛（Anacardiaceae）的遗传结构，多样性和基因流动模式的影响。利用基因组文库富集，开发了十六种微卫星基因座。在来自墨西哥四个地区的80位个体中评估了多态性。检测到的等位基因总数为63，每个基因座的平均等位基因为3.9，范围为每个基因座1至11。在漆树科的相关物种上的交叉扩增轨迹：Spondiaspurpurea和Amphipterygiumadstringens，已成功扩增所有引物。这里描述的微卫星标记是第一个被鉴定为A.graveolens的标记。这些遗传标志物将是评估生境破碎化和对A.graveolens种群进行选择性伐木的遗传后果的有用工具。S.purpureaandA.adstringens的交叉扩增成功表明，它们可用于该家族其他物种的种群遗传研究。