当前位置: X-MOL 学术Biol. Invasions › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Apple snail egg perivitellin coloration, as a taxonomic character for invasive Pomacea maculata and P. canaliculata, determined by a simple method
Biological Invasions ( IF 2.9 ) Pub Date : 2020-04-02 , DOI: 10.1007/s10530-020-02255-z
M. Y. Pasquevich , H. Heras

Abstract

Pomacea canaliculata and P. maculata are freshwater apple snails considered two of the most invasive alien species worldwide. They are expanding their ranges in Asia, Europe and North America, damaging crops, wetland ecosystems and endangering human health. Despite having different invasive potential, they have systematically been misidentified because of their morphological plasticity with highly variable shells, which hampers efforts to manage their spread and impact. Thus, additional work is needed to clarify species boundaries in this group of Pomacea. Here we report a new taxonomic character based on the spectroscopic characteristics of their pigmented perivitellins (egg proteins) and a fast and simple method for measuring it. The egg perivitelline fluid is analyzed via conventional agarose gel electrophoresis and the naturally-colored egg carotenoproteins are extracted, without the need for staining, and analyzed spectrophotometrically. Then, the ratio between two wavelengths (PQ) of the absorbance spectra is calculated. Results can be obtained in less than 24 h. The method was validated in the field by analysing seven populations from Asia (non-native) and South America (native) that had been genetically identified and in which PQ differed consistently between the two species. Eggs are oviposited in conspicuous reddish masses above the waterline, allowing rapid detection and easy collection of samples in the field. This novel tool would be valuable in integrative taxonomic studies to distinguish these two lineages of Pomacea and contribute to our understanding of Pomacea diversity.

Graphic Abstract



中文翻译:

苹果蜗牛卵皮蛋白的着色,作为入侵性Po果和小菜蛾的分类学特征,可通过简单方法测定

摘要

Pomacea canaliculataP. maculata是淡水苹果蜗牛,被认为是全球入侵性最高的两种外来物种。他们正在扩大在亚洲,欧洲和北美洲的范围,损害作物,湿地生态系统并危及人类健康。尽管具有不同的侵袭潜力,但由于它们的形态可塑性和高度可变的外壳,因此系统地将它们误认为是错误的,这阻碍了管理其扩散和影响的努力。因此,需要做更多的工作来弄清这一类Pomacea中的物种边界。在这里,我们报告了基于色素的玻璃素蛋白(鸡蛋蛋白)的光谱特征的新分类学特征,以及一种快速简单的测量方法。通过常规的琼脂糖凝胶电泳对卵周质进行分析,无需染色即可提取天然颜色的卵胡萝卜素蛋白,并进行分光光度法分析。然后,计算吸收光谱的两个波长之间的比率(PQ)。不到24小时即可获得结果。该方法通过分析来自亚洲(非本地)和南美(本地)的七个种群进行了实地验证,这七个种群已经过基因鉴定,并且两个物种之间的PQ始终相同。卵在水线上方显着的淡红色卵生,可以在现场快速检测并轻松收集样品。这种新颖的工具在整合生物分类学研究中将有助于区分这两个世系。Pomacea有助于我们对Pomacea多样性的理解。

图形摘要

更新日期:2020-04-20
down
wechat
bug