Nowadays, analytical techniques are moving towards the development of smart biosensing strategies for the point-of-care accurate screening of disease biomarkers, such as human epididymis protein 4 (HE4), a recently discovered serum marker for early ovarian cancer diagnosis. In this context, the present work represents the first implementation of a competitive enzyme-labelled magneto-immunoassay exploiting a homemade IoT Wi-Fi cloud-based portable potentiostat for differential pulse voltammetry readout. The electrochemical device was specifically designed to be capable of autonomous calibration and data processing, switching between calibration, and measurement modes: in particular, firstly, a baseline estimation algorithm is applied for correct peak computation, then calibration function is built by interpolating data with a four-parameter logistic function. The calibration function parameters are stored on the cloud for inverse prediction to determine the concentration of unknown samples. Interpolation function calibration and concentration evaluation are performed directly on-board, thus reducing the power consumption. The analytical device was validated in human serum, demonstrating good sensing performance for analysis of HE4 with detection and quantitation limits in human serum of 3.5 and 29.2 pM, respectively, reaching the sensitivity that is required for diagnostic purposes, with high potential for applications as portable and smart diagnostic tool for point-of-care testing.

中文翻译：

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用于血清人附睾蛋白4的自校准IoT便携式电化学免疫传感器，作为卵巢癌的肿瘤生物标记物。

如今，分析技术正朝着智能生物传感策略的发展方向发展，以便在现场即时准确筛查疾病生物标记物，例如人类附睾蛋白4（HE4），后者是最近发现的用于卵巢癌早期诊断的血清标记物。在这种情况下，本工作代表了一种竞争性酶标记磁免疫测定法的首次实施，该方法利用自制的基于物联网Wi-Fi云的便携式恒电位仪进行差分脉冲伏安法读数。电化学设备经过专门设计，能够进行自动校准和数据处理，在校准和测量模式之间切换：特别是，首先，将基线估计算法应用于正确的峰计算，然后通过使用四参数逻辑函数对数据进行插值来构建校准函数。校准功能参数存储在云中，用于逆向预测以确定未知样品的浓度。插值功能校准和浓度评估直接在板上进行，从而降低了功耗。该分析仪已在人血清中进行了验证，证明其具有良好的传感性能，可对HE4进行分析，其人血清中的检测和定量限分别为3.5和29.2 pM，达到了诊断目的所需的灵敏度，在便携式应用中具有很高的潜力以及用于现场测试的智能诊断工具。校准功能参数存储在云中，用于逆向预测以确定未知样品的浓度。插值功能校准和浓度评估直接在板上进行，从而降低了功耗。该分析仪已在人血清中进行了验证，证明其具有良好的传感性能，可对HE4进行分析，其人血清中的检测和定量限分别为3.5和29.2 pM，达到了诊断目的所需的灵敏度，在便携式应用中具有很高的潜力以及用于现场测试的智能诊断工具。校准功能参数存储在云中，用于逆向预测以确定未知样品的浓度。插值功能校准和浓度评估直接在板上进行，从而降低了功耗。该分析仪已在人血清中进行了验证，证明其具有良好的传感性能，可对HE4进行分析，其人血清中的检测和定量限分别为3.5和29.2 pM，达到了诊断目的所需的灵敏度，在便携式应用中具有很高的潜力以及用于现场测试的智能诊断工具。