Different parts of Nuphar lutea L. (yellow water lily) have been used to treat several inflammatory and pathogen-related diseases. It has shown that Nuphar lutea extracts (NUP) are active against various pathogens including bacteria, fungi, and leishmanial parasites. In an effort to detect novel therapeutic agents against negative-stranded RNA (- RNA) viruses, we have tested the effect of a partially-purified alkaloid mixture of Nuphar lutea leaves on the measles virus (MV). The MV vaccine’s Edmonston strain was used to acutely or persistently infect cells. The levels of several MV proteins were detected by a Western blot and immunocytochemistry. Viral RNAs were quantitated by qRT-PCR. Virus infectivity was monitored by infecting African green monkey kidney VERO cells’ monolayers. We showed that NUP protected cells from acute infection. Decreases in the MV P-, N-, and V-proteins were observed in persistently infected cells and the amount of infective virus released was reduced as compared to untreated cells. By examining viral RNAs, we suggest that NUP acts at the post-transcriptional level. We conclude, as a proof of concept, that NUP has anti-viral therapeutic activity against the MV. Future studies will determine the mechanism of action and the effect of NUP on other related viruses.

中文翻译：

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Nuphar lutea 提取物对麻疹病毒具有抗病毒活性

Nuphar lutea L.（黄色睡莲）的不同部分已被用于治疗多种炎症和病原体相关疾病。研究表明，Nuphar lutea 提取物 (NUP) 对各种病原体具有活性，包括细菌、真菌和利什曼原虫寄生虫。为了检测针对负链 RNA (- RNA) 病毒的新型治疗剂，我们测试了部分纯化的黄芪叶生物碱混合物对麻疹病毒 (MV) 的影响。MV 疫苗的 Edmonston 菌株用于急性或持续感染细胞。通过蛋白质印迹和免疫细胞化学检测几种MV蛋白的水平。通过 qRT-PCR 对病毒 RNA 进行定量。通过感染非洲绿猴肾 VERO 细胞的单层来监测病毒感染性。我们发现 NUP 保护细胞免受急性感染。与未处理的细胞相比，在持续感染的细胞中观察到 MV P-、N-和 V-蛋白的减少，并且释放的感染病毒的量减少。通过检查病毒 RNA，我们建议 NUP 在转录后水平起作用。我们得出结论，作为概念证明，NUP 具有针对 MV 的抗病毒治疗活性。未来的研究将确定 NUP 对其他相关病毒的作用机制和影响。