Preparation of biodiesel using in situ transesterification has been extensively conducted for agricultural, microbial and algal biomass, while few works have been performed using aquatic animal tissue. In this work, fish processing wastes were collected to perform in situ transesterification using grass carp (Ctenopharyngodon idellus) biomass as a representative with which to optimize the reaction conditions. Under the optimum condition, the highest biodiesel purity reached up to 100% for sea bass wastes, which is higher than the 96.5% specified in the EN 14214-2008. The in situ method proposed here has the potential to save significant costs in biodiesel production compared to conventional methods, which usually require high-cost pretreatment of the raw materials. Additionally, the waste residue byproduct produced has a high protein content, and therefore the potential to be used for high-protein feed. This study is expected to inspire new strategies to prepare biodiesel and high-protein feed simultaneously from aquatic animal biomass using the novel in situ transesterification.

中文翻译：

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使用原位酯交换从鱼类加工废物生产生物柴油和高蛋白饲料

使用原位酯交换制备生物柴油已广泛用于农业、微生物和藻类生物质，而使用水生动物组织进行的工作很少。在这项工作中，收集鱼类加工废物以使用草鱼（Ctenopharyngodon idellus）生物质进行原位酯交换，以优化反应条件。在最佳条件下，鲈鱼废物的最高生物柴油纯度可达100%，高于EN 14214-2008规定的96.5%。与通常需要对原材料进行高成本预处理的传统方法相比，这里提出的原位方法有可能在生物柴油生产中节省大量成本。此外，产生的废渣副产品蛋白质含量高，因此有可能用于高蛋白饲料。这项研究有望激发新的策略，使用新颖的原位酯交换从水生动物生物质中同时制备生物柴油和高蛋白饲料。