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An FMRFamide Neuropeptide in Cuttlefish Sepia pharaonis: Identification, Characterization, and Potential Function
Molecules ( IF 4.6 ) Pub Date : 2020-04-02 , DOI: 10.3390/molecules25071636
Yang Zhu 1 , Lian-Lian Sun 1 , Jun-Hong Wu 1 , Hui-Hui Liu 1 , Li-Bing Zheng 1 , Zhen-Ming Lü 1 , Chang-Feng Chi 1
Affiliation  

Neuropeptides are released by neurons that are involved in a wide range of brain functions, such as food intake, metabolism, reproduction, and learning and memory. A full-length cDNA sequence of an FMRFamide gene isolated from the cuttlefish Sepia pharaonis (designated as SpFMRFamide) was cloned. The predicted precursor protein contains one putative signal peptide and four FMRFamide-related peptides. Multiple amino acid and nucleotide sequence alignments showed that it shares 97% similarity with the precursor FMRFamides of Sepiella japonica and Sepia officinalis and shares 93% and 92% similarity with the SpFMRFamide gene of the two cuttlefish species, respectively. Moreover, the phylogenetic analysis also suggested that SpFMRFamide and FMRFamides from S. japonica and S. officinalis belong to the same sub-branch. Tissue expression analysis confirmed that SpFMRFamide was widely distributed among tissues and predominantly expressed in the brain at the three development stages. The combined effects of SpFMRFamide+SpGnRH and SpFLRFamide+SpGnRH showed a marked decrease in the level of the total proteins released in the CHO-K1 cells. This is the first report of SpFMRFamide in S. pharaonis and the results may contribute to future studies of neuropeptide evolution or may prove useful for the development of aquaculture methods for this cuttlefish species.

中文翻译:

墨鱼棕褐色法老鱼中的 FMRFamide 神经肽:鉴定、表征和潜在功能

神经肽由参与广泛大脑功能的神经元释放,例如食物摄入、新陈代谢、繁殖以及学习和记忆。克隆了从乌贼乌贼棕褐色(指定为 SpFMRFamide)中分离的 FMRFamide 基因的全长 cDNA 序列。预测的前体蛋白包含一种推定的信号肽和四种 FMRFamide 相关肽。多个氨基酸和核苷酸序列比对表明,它与乌贼属和乌贼属的前体 FMRFamides 具有 97% 的相似性,与两种墨鱼物种的 SpFMRFamide 基因分别具有 93% 和 92% 的相似性。此外,系统发育分析还表明,来自S. japonica 和S. officinalis 的SpFMRFamide 和FMRFamides 属于同一分支。组织表达分析证实,SpFMRFamide 广泛分布于组织中,并在三个发育阶段主要在大脑中表达。SpFMRFamide + SpGnRH 和 SpFLRFamide + SpGnRH 的联合作用显示 CHO-K1 细胞中释放的总蛋白质水平显着降低。这是 S. pharaonis 中 SpFMRFamide 的第一份报告,结果可能有助于神经肽进化的未来研究,或者可能证明对开发这种墨鱼物种的水产养殖方法有用。
更新日期:2020-04-02
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