Obesity and menopause are known as a major risk factor in the development of left ventricular (LV) dysfunction. Calorie restriction (CR) or exercise (Ex) improved metabolic status and LV function. This study aims to investigate the combined effects of Ex and CR on the cardiometabolic status, and cardiac calcium ([Ca2+]i) regulation in estrogen-deprivation, obese prediabetic rats. Female rats were fed with either a high-fat diet (HFD) or a normal diet for 13 weeks. The HFD rats were ovariectomized (HFO), and subjected to 1) vehicle (HFOV); 2) calorie restriction (HFOCR); 3) exercise (HFOEx); 4) combined therapy (HFOCB); or 5) estrogen (HFOE2). After six weeks of interventions the cardiometabolic status, cardiac [Ca2+]i transients, mitochondrial function and dynamics were determined. HFD-fed rats developed insulin resistance as indicated by increased plasma insulin and HOMA index. Although rats in the HFOV groups had markedly reduced %LVFS which indicated impaired LV function, impaired [Ca2+]i homeostasis, cardiac mitochondrial function and their dynamics, all interventions attenuated these impairments. Interestingly, HFOCB rats were observed to have the greatest cardiometabolic improvement. The combination of calorie restriction and exercise exerted greater efficacy in attenuating LV dysfunction through an improved metabolic status, cardiac function, mitochondrial function, and cardiac [Ca2+]i homeostasis than Ex or CR monotherapy in ovariectomized obese prediabetic rats.

中文翻译：

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限制卡路里的运动通过减轻卵巢切除的糖尿病前期大鼠的线粒体功能障碍来改善心脏功能。

肥胖和更年期是导致左心室（LV）功能障碍的主要危险因素。热量限制（CR）或运动（Ex）改善了代谢状态和LV功能。这项研究旨在探讨Ex和CR对肥胖，肥胖的糖尿病前期大鼠心脏代谢状态和心脏钙（[Ca2 +] i）调节的综合影响。给雌性大鼠喂高脂饮食（HFD）或正常饮食13周。将HFD大鼠切除卵巢（HFO），并进行1）媒介物（HFOV）；2）卡路里限制（HFOCR）；3）锻炼（HFOEx）；4）联合治疗（HFOCB）；或5）雌激素（HFOE2）。经过六周的干预，确定了心脏的代谢状态，心脏[Ca2 +] i瞬变，线粒体功能和动力学。用HFD喂养的大鼠出现胰岛素抵抗，如血浆胰岛素和HOMA指数升高所表明。尽管HFOV组的大鼠％LVFS明显降低，这表明LV功能受损，[Ca2 +] i体内稳态受损，心脏线粒体功能及其动力学变化，但所有干预措施均可减轻这些损害。有趣的是，观察到HFOCB大鼠具有最大的心脏代谢改善。与Ex或CR单一疗法相比，卡路里限制和运动相结合的方法通过改善代谢状态，心脏功能，线粒体功能和心脏[Ca2 +] i稳态而在减轻LV功能障碍方面发挥了更大的功效，而在卵巢切除的肥胖前期糖尿病大鼠中，这种疗法的效果优于Ex或CR单一疗法。[Ca2 +] i稳态受损，心脏线粒体功能及其动力学受损，所有干预措施均可减轻这些损害。有趣的是，观察到HFOCB大鼠的心脏代谢改善最大。与Ex或CR单一疗法相比，卡路里限制和运动相结合的方法通过改善代谢状态，心脏功能，线粒体功能和心脏[Ca2 +] i稳态而在减轻LV功能障碍方面发挥了更大的功效，而在卵巢切除的肥胖前期糖尿病大鼠中，这种疗法的效果优于Ex或CR单一疗法。[Ca2 +] i稳态受损，心脏线粒体功能及其动力学受损，所有干预措施均可减轻这些损害。有趣的是，观察到HFOCB大鼠的心脏代谢改善最大。与Ex或CR单一疗法相比，卡路里限制和运动相结合的方法通过改善代谢状态，心脏功能，线粒体功能和心脏[Ca2 +] i稳态而在减轻LV功能障碍方面发挥了更大的功效，而在卵巢切除的肥胖前期糖尿病大鼠中，这种疗法的效果优于Ex或CR单一疗法。