A critical factor for the performance of a biofuel cell is an immobilization of the redox enzyme for continuous catalytic reaction and efficient electron transfer. However, the main obstacle associated with enzyme electrode is the reduced surface area for the accommodation of enzymes, leading to poor power output. This study aimed to optimize the efficient electrical communication for glucose oxidase (GOx) on the surface of a graphite oxide/cobalt hydroxide/chitosan composite as mediator, thereby enhancing the generation of power output. Immobilization efficiency was affected by the different concentrations of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS). Also, the surface of enzyme electrode was observed by XPS, Raman, and AFM, respectively. The electrochemical characterization showed that the immobilized GOx possesses the highest activity at EDC:NHS(40:80 mM) concentration. The power output under the optimal condition was found to be 2.24 mWcm⁻² of power density using the three-electrode cell in 0.1 M PBS solution at room temperature.

生物燃料电池性能的关键因素是氧化还原酶的固定化，以实现连续的催化反应和有效的电子转移。然而，与酶电极相关的主要障碍是用于容纳酶的表面积减小，从而导致不良的功率输出。这项研究旨在优化作为介体的氧化石墨/氢氧化钴/壳聚糖复合材料表面的葡萄糖氧化酶（GOx）的有效电通信，从而提高功率输出的产生。固定效率受1-乙基-3-（3-二甲基氨基丙基）-碳二亚胺（EDC）/ N-羟基琥珀酰亚胺（NHS）不同浓度的影响。另外，分别通过XPS，拉曼和AFM观察酶电极的表面。电化学表征表明，固定化的GOx在EDC：NHS（40:80 mM）浓度下具有最高的活性。发现在最佳条件下的功率输出为2.24 mWcm在室温下，使用三电极电池在0.1 M PBS溶液中的功率密度^-2。