Astragalus is a genus with many important medicinal plant species. The historical background of Astragalus species dates back to 2000–3000 years. In this study, it was aimed to investigate the phytochemical composition, antioxidant, tyrosinase and α-amylase inhibitory activities of the MeOH (methanol) extracts obtained from three different Astragalus species (A. gymnolobus Fisch., A. leporinus Boiss. var. hirsutus (Post) D.F. Chamb., and A. onobrychis L.). It was understood that A. gymnolobus and A. onobrychis extracts are richer than A. leporinus var. hirsutusin terms of phenolics and flavonoids. According to the results of Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC–ESI–MS/MS) analysis, it was determined that hesperidin and hyperoside were found in high amounts in the extracts. A strong correlation was found between the phytochemical compositions of the plants and their antioxidant activities. The order of the antioxidant activity of A. gymnolobus from strongest to the weakest was as follows: Ferric Reducing Ability of Plasma (FRAP) (1.13 mg/mL), ferrous ion chelating (1.53 mg/mL), Cupric Reducing Antioxidant Capacity (CUPRAC) (1.58 mg/mL), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) (2.52 mg/mL), phosphomolybdenum (2.91 mg/mL), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) (3.86 mg/mL). In the case of A. onobrychis, the order of the antioxidant activity was found to be: FRAP (1.25 mg/mL), ferrous ion chelating assays (1.69 mg/mL), CUPRAC (1.93 mg/mL), ABTS (2.21 mg/mL), phosphomolybdenum (2.77 mg/mL), and DPPH (4.50 mg/mL). A. gymnolobus (1.27 mg/mL, 281.92 mg KAEs/g extract) and A. onobrychis (1.25 mg/mL, 287.89 mg KAEs/g extract) have also been found to be effective tyrosinase inhibitors. In α-amylase inhibitory activity test, A. leporinus var. hirsutus was found to show the highest activity (4.06 mg/mL, 298.54 mg ACEs/g extract). According to Pearson correlation test results, it was concluded that hesperidin significantly contributed to the biological activities of the extracts.

黄芪是许多重要药用植物的属。黄芪物种的历史背景可以追溯到2000-3000年。在这项研究中，它旨在探讨植物化学物质组合物，抗氧化剂，酪氨酸酶和α淀粉酶从三个不同的获得的MeOH（甲醇）提取物的抑制活性黄芪物种（A. gymnolobus甘草。，A. leporinus羌活。变种毛云芝菌（邮政）DF Chamb。和A. onobrychis L.）。据了解，A。Gymnolobus和A. onobrychis提取物比A. leporinus var丰富。hirsutus就酚类和类黄酮而言。根据液相色谱电喷雾串联质谱法（LC-ESI-MS / MS）的分析结果，可以确定提取物中含有大量的橙皮苷和金丝桃苷。发现植物的植物化学组成与其抗氧化活性之间存在很强的相关性。裸枝线虫的抗氧化活性从强到弱的顺序如下：血浆铁还原能力（FRAP）（1.13 mg / mL），亚铁离子螯合（1.53 mg / mL），铜还原抗氧化能力（CUPRAC） ）（1.58 mg / mL），2,2'-叠氮基双-3-乙基苯并噻唑啉-6-磺酸（ABTS）（2.52 mg / mL），磷钼（2.91 mg / mL）和1,1-二苯基- 2-吡啶甲基肼基（DPPH）（3.86 mg / mL）。如果是洋葱on（A. onobrychis）的抗氧化活性顺序为：FRAP（1.25 mg / mL），亚铁离子螯合测定（1.69 mg / mL），CUPRAC（1.93 mg / mL），ABTS（2.21 mg / mL），磷钼（2.77 mg / mL）和DPPH（4.50 mg / mL）。业已发现，裸子线虫（1.27 mg / mL，281.92 mg KAEs / g提取物）和Onobrychis（1.25 mg / mL，287.89 mg KAEs / g提取物）是有效的酪氨酸酶抑制剂。在α-淀粉酶抑制活性试验中，A。leporinus var。发现hirsutus表现出最高活性（4.06 mg / mL，298.54 mg ACEs / g提取物）。根据皮尔森相关性测试结果，可以得出结论，橙皮苷对提取物的生物活性有重要贡献。