Phthalate ester (PAE) pollution is an increasing problem globally. Paracoccus kondratievae BJQ0001 was isolated from the fermentation starter of Baijiu and showed an efficient degradation capability toward PAEs. To our poor knowledge, this is the first report of a P. kondratievae strain capable of degrading PAEs. The first complete genome sequence of P. kondratievae was presented without gaps, and composed of two circular chromosomes and one plasmid. The species simultaneously degraded di-methyl phthalate (DMP), di-ethyl phthalate (DEP), di-butyl phthalate (DBP), di-isobutyl phthalate (DIBP) and di-(2-ethylhexyl) phthalate (DEHP), with DMP and DEP as the preferred substrates. The half-life (t1/2) of DMP was only 6.34 h with an initial concentration of 200 mg/L. Combined with gene annotation and metabolic intermediate analysis, a metabolic pathway was proposed for the species. Benzoic acid, the intermediate of anaerobic PAE metabolism, was identified in the aerobic degradation process. Two key enzymes for alkyl ester bond hydrolysis were obtained, and belonged to families IV and VI of hydrolases, respectively. These results will promote the investigation of PAE degradation by P. kondratievae, and provide useful information for improving the quality control of food and environmental PAE treatment.

中文翻译：

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从酒九（白酒发酵发酵剂）中分离得到的副球菌BJQ0001对邻苯二甲酸酯进行生物降解，并鉴定酯键水解酶。

邻苯二甲酸酯（PAE）污染是全球范围内日益严重的问题。从白酒的发酵发酵液中分离出康氏副球菌BJQ0001，并表现出对PAEs的有效降解能力。据我们所知，这是能够降解PAEs的康氏疟原虫菌株的首次报道。第一个完整的P. kondratievae基因组序列完整无缺，由两个环形染色体和一个质粒组成。该物种同时使用DMP降解了邻苯二甲酸二甲酯（DMP），邻苯二甲酸二乙酯（DEP），邻苯二甲酸二丁酯（DBP），邻苯二甲酸二异丁酯（DIBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP）和DEP作为首选基材。DMP的半衰期（t1 / 2）仅6.34小时，初始浓度为200 mg / L。结合基因注释和代谢中间分析，提出了该物种的代谢途径。苯甲酸是厌氧PAE代谢的中间产物，在需氧降解过程中得到鉴定。获得了两个用于烷基酯键水解的关键酶，分别属于水解酶的IV和VI族。这些结果将促进对P. kondratievae对PAE降解的研究，并为改善食品和环境PAE处理的质量控制提供有用的信息。