Highly selective adsorption of protein species interested from complicated biological samples is an extremely important prerequisite for in-depth proteomic analysis. Herein, a novel dual-hydrophilic material is fabricated by grafting Zr-based metal-organic framework (Zr-MOF) onto the surface of magnetic microspheres, and subsequent modifying with fructose-1,6-bisphosphate (FBP) molecules by taking advantage of multivalent binding between the phosphate backbone of FBP and Zr centers. Owing to the favorable dual-hydrophilicity derived from the abundant amino groups in Zr-MOF and the hydroxyl groups in FBP molecules, the obtained magnetic microspheres (mMOF-FBP) provide high adsorption efficiency, favorable selectivity and excellent capturing capacity (555.6 mg g-1) towards immunoglobulin G (IgG). The captured IgG could be collected from the mMOF-FBP by using 0.5% (m/v) NH3·H2O as elution reagent with a recovery of 88.4%. The dual-hydrophilic microspheres demonstrate their practicability by selective isolation of IgG from human serum.

中文翻译：

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双磷酸化果糖修饰的磁性 Zr 有机框架：一种用于选择性吸附免疫球蛋白 G 的双亲水吸附剂

对复杂生物样品中感兴趣的蛋白质种类的高选择性吸附是深入蛋白质组学分析的一个极其重要的先决条件。在此，通过将 Zr 基金属有机骨架 (Zr-MOF) 接枝到磁性微球表面，然后利用 1,6-二磷酸果糖 (FBP) 分子修饰，制备了一种新型双亲水材料。 FBP 的磷酸骨架和 Zr 中心之间的多价结合。由于Zr-MOF中丰富的氨基和FBP分子中的羟基具有良好的双重亲水性，所获得的磁性微球（mMOF-FBP）具有高吸附效率、良好的选择性和优异的捕获能力（555.6 mg g- 1) 对免疫球蛋白 G (IgG)。使用0.5% (m/v) NH3·H2O作为洗脱剂，可以从mMOF-FBP中收集捕获的IgG，回收率为88.4%。双亲水微球通过从人血清中选择性分离 IgG 来证明其实用性。