Abstract Tetracycline and pyrethroid residues can reach food of animal origin such as milk due to their multiple uses in modern systems of intensive breeding. Thus, regulatory entities established the maximum residue limit (MRL) for these contaminants to guarantee food safety. Despite that, impact derived from simultaneous presence (cocktail effect) of tetracycline and pyrethroid residues on food proteins is unknown. This study evaluated oxidative damage promoted on caseins and whey proteins by the binary mixture of four tetracyclines with three pyrethroids at respective maximum residue limit values using proteomic techniques. Casein and whey proteins exhibited significant solubility loss and suffered irreversible oxidative damage relative to control samples in eleven of twelve binary combinations of tetracyclines and pyrethroids assayed. Based on the magnitude of solubility loss and carbonylation induced by tetracycline and pyrethroid mixtures on milk proteins, new concerns are opened around the real innocuity of MRL for these substances.

中文翻译：

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四环素和拟除虫菊酯残基的组合增加了牛奶中的蛋白质羰基化

摘要 四环素和拟除虫菊酯残留物在现代集约化养殖系统中具有多种用途，因此可以接触到动物源性食品，如牛奶。因此，监管实体为这些污染物制定了最大残留限量 (MRL)，以保证食品安全。尽管如此，由于四环素和拟除虫菊酯残留物同时存在（鸡尾酒效应）对食物蛋白质的影响尚不清楚。本研究使用蛋白质组学技术评估了四种四环素和三种拟除虫菊酯的二元混合物在各自的最大残留限值下对酪蛋白和乳清蛋白的氧化损伤。在所分析的 12 种四环素和拟除虫菊酯二元组合中的 11 种中，酪蛋白和乳清蛋白表现出显着的溶解度损失并遭受相对于对照样品的不可逆氧化损伤。