当前位置:
X-MOL 学术
›
J. Biotechnol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Increasing L-homoserine production in Escherichia coli by engineering the central metabolic pathways.
Journal of Biotechnology ( IF 4.1 ) Pub Date : 2020-04-03 , DOI: 10.1016/j.jbiotec.2020.03.010 Min Liu 1 , Jiali Lou 1 , Jiali Gu 2 , Xiao-Mei Lyu 3 , Feng-Qing Wang 1 , Dong-Zhi Wei 1
Journal of Biotechnology ( IF 4.1 ) Pub Date : 2020-04-03 , DOI: 10.1016/j.jbiotec.2020.03.010 Min Liu 1 , Jiali Lou 1 , Jiali Gu 2 , Xiao-Mei Lyu 3 , Feng-Qing Wang 1 , Dong-Zhi Wei 1
Affiliation
l-homoserine is an important functional amino acid. Based on the system metabolic engineering strategy, the key genes in the central metabolic pathway of Escherichia coli W3110 were engineered to construct the strain for l-homoserine production. To construct an engineered strain with high yield of l-homoserine, the work was carried out from the following aspects: (1) Disrupt the competitive and degradative pathways of l-homoserine, and the l-homoserine was initially accumulated with a titer of 0.2 g/L; (2) Exploring the effect of weakening TCA cycle, modification of the glyoxylate branch, and reduction of the pyruvate synthesis for l-homoserine synthesis. The concentration of l-homoserine in the final recombinant strain LJL12 reached a titer of 3.2 g/L at shake flask and 35.8 g/L in fed-batch fermentation, showing a high l-homoserine production capacity (0.82 g/L/h). The study provides a well research foundation for l-homoserine production with the capacity for industrial application.
中文翻译:
通过设计中央代谢途径来增加大肠杆菌中L-高丝氨酸的产量。
l-高丝氨酸是一种重要的功能氨基酸。基于系统代谢工程学策略,对大肠杆菌W3110中央代谢途径中的关键基因进行了工程改造,以构建用于生产L-高丝氨酸的菌株。为了构建高产量的左旋高丝氨酸工程菌,从以下几个方面进行了研究:(1)破坏左旋高丝氨酸的竞争和降解途径,最初以浓度为0.2的方式积累了左旋高丝氨酸。克/升 (2)探索弱化TCA循环,修饰乙醛酸支链和减少丙酮酸合成的合成现象。最终重组菌株LJL12中的l-高丝氨酸浓度在摇瓶中达到3.2 g / L,在分批补料发酵中达到35.8 g / L,显示出高的高丝氨酸生产能力(0.82 g / L / h)。该研究为具有工业应用能力的左旋高丝氨酸生产提供了良好的研究基础。
更新日期:2020-04-21
中文翻译:
通过设计中央代谢途径来增加大肠杆菌中L-高丝氨酸的产量。
l-高丝氨酸是一种重要的功能氨基酸。基于系统代谢工程学策略,对大肠杆菌W3110中央代谢途径中的关键基因进行了工程改造,以构建用于生产L-高丝氨酸的菌株。为了构建高产量的左旋高丝氨酸工程菌,从以下几个方面进行了研究:(1)破坏左旋高丝氨酸的竞争和降解途径,最初以浓度为0.2的方式积累了左旋高丝氨酸。克/升 (2)探索弱化TCA循环,修饰乙醛酸支链和减少丙酮酸合成的合成现象。最终重组菌株LJL12中的l-高丝氨酸浓度在摇瓶中达到3.2 g / L,在分批补料发酵中达到35.8 g / L,显示出高的高丝氨酸生产能力(0.82 g / L / h)。该研究为具有工业应用能力的左旋高丝氨酸生产提供了良好的研究基础。