Abstract Two studies were conducted with objective of determine the specific requirements of arginine (Arg) for maintenance using different unit systems; evaluate the response of broiler breeder hens of Ross 308AP genotype to different intakes of arginine, determine amino acid utilization efficiency (k), and develop a factorial model. One nitrogen balance trial (Experiment 1) was performed using 42 roosters and another dose response trial was performed using 64 broiler breeder hens (Experiment 2). The nitrogen balance trial lasted five days with 48 h of fasting (with roosters receiving only water + sucrose) and the last 72 h for feeding and excreta collection. Forty grams of each diet first-limiting in arginine was fed by tube each day (three days) to give a range of intakes from 0 to 18.5 g/kg BW d of arginine. Excreta were collected during the last 3 days of the balance period and the nitrogen content of the excreta was analyzed. A linear regression between nitrogen retention (NR) and arginine intake was performed. The studies were conducted in a complete random design. Were used with 6 treatments, 6 replicates for Experiment 1 and, 8 treatments with 6 replicates for Experiment 2, and one bird per cage. To confirm that the amino acid studied was indeed limiting and that the responses were due to the limitation of the test amino acid and not protein, we added one more treatment consisting of a control diet (totaling 7 treatments for Experiment 1 and 8 treatments for Experiment 2). The diets were formulated by dilution technique using one summit diet and one nitrogen (N)-free diet, resulting in AA levels that ranged from 2.37 to 10.27 g of arginine/kg. The data obtained were AA intake (AAI), body weight (BW), and egg output (EO). Broken line model was used to evaluate the responses. The equation from linear regression was: NR = -39.9 (± 4.5) + 1.8 (± 0.1) × Arg. The arginine required to maintain the body at zero NR were calculated to be 23 mg/kg body weight per day, respectively. For the system unit mg per kg of metabolic weight, the intake of arginine was 36. Considering the degree of maturity of the animal and body protein content the amounts of arginine required for maintenance was calculated to be 151 mg per unit of maintenance protein (BP m0.73 × u) per day. The values estimated by the model for utilization efficiency was 62 %. The arginine requirement estimated by the model at 30 weeks was 753 mg/day. The prediction of the model was improved using the coefficients estimated here with physiologically relevant units.

中文翻译：

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肉鸡种鸡维持和产蛋所需的精氨酸要求

摘要 进行了两项研究，目的是确定使用不同单位系统进行维护的精氨酸 (Arg) 的具体要求；评估罗斯 308AP 基因型肉种鸡对不同精氨酸摄入量的反应，确定氨基酸利用效率 (k)，并建立因子模型。使用 42 只公鸡进行了一项氮平衡试验（实验 1），使用 64 只肉种鸡进行了另一项剂量反应试验（实验 2）。氮平衡试验持续 5 天，禁食 48 小时（公鸡仅接受水 + 蔗糖），最后 72 小时用于喂养和排泄物收集。每天（三天）通过试管饲喂 40 克精氨酸第一限制的每种饮食，以提供 0 到 18.5 g/kg BW d 的精氨酸摄入量。在平衡期的最后 3 天收集排泄物并分析排泄物的氮含量。进行了氮保留 (NR) 和精氨酸摄入量之间的线性回归。这些研究是在完全随机设计中进行的。使用 6 个处理，实验 1 6 个重复，8 个处理，实验 2 6 个重复，每笼一只鸟。为了确认研究的氨基酸确实是限制性的，并且反应是由于测试氨基酸而不是蛋白质的限制，我们又添加了一种由对照饮食组成的处理（实验 1 总共 7 个处理，实验 1 的 8 个处理） 2）。日粮是通过稀释技术使用一种顶峰日粮和一种无氮 (N) 日粮配制的，导致 AA 水平范围为 2.37 至 10.27 克精氨酸/公斤。获得的数据是 AA 摄入量 (AAI)、体重 (BW) 和产蛋量 (EO)。折线模型用于评估响应。线性回归方程为：NR = -39.9 (± 4.5) + 1.8 (± 0.1) × Arg。将身体维持在零 NR 所需的精氨酸分别计算为每天 23 毫克/公斤体重。对于系统单位mg/kg代谢重，精氨酸的摄入量为36。考虑到动物的成熟程度和体内蛋白质含量，计算维持所需的精氨酸量为151mg/单位维持蛋白质（BP m0.73 × u) 每天。该模型估计的利用效率值为 62%。该模型在 30 周时估计的精氨酸需要量为 753 毫克/天。