In this study, an ultrasensitive electrochemical aptasensor for the determination of the β-casomorphin 7 (BCM-7) as a promising biomarker of autism disorder is introduced. According to the proposed strategy, a glassy carbon electrode (GCE) was modified by thiourea capped-ZnS QDs (ZnS-QDs) and gold nanoparticles (AuNPs) to further immobilization the amino-aptamer (NH₂-Apt) on its surface. The NH₂-Apt as a receptor of the BCM-7 was attached to the embedded surface via a formation of the Au–N bonds between the AuNPs and amino groups of the Apt. The evaluation of aptasensor by various electrochemical techniques exhibited successful sensing of the BCM-7 under two broad linear concentration ranges from 1 fM to 0.6 μM with a limit of detection (LOD) down to 350 aM. Also, the performance of the aptasensor in BCM-7 detection in real human urine samples was satisfactorily investigated. These findings may facilitate distinguish of this challenging disorder and help human health.

在这项研究中，介绍了一种用于测定β-casomorphin7（BCM-7）作为自闭症有希望的生物标志物的超灵敏电化学适体传感器。根据提出的策略，通过硫脲封端的ZnS QD（ZnS-QDs）和金纳米颗粒（AuNPs）修饰玻璃碳电极（GCE），以进一步将氨基适体（NH ₂ -Apt）固定在其表面上。NH ₂-Apt作为BCM-7的受体通过AuNPs和Apt氨基之间的Au-N键形成而附着在嵌入式表面上。通过各种电化学技术对适体传感器进行的评估显示，在从1 fM到0.6μM的两个宽线性浓度范围内，对BCM-7的成功检测，检测限（LOD）降至350 aM。另外，令人满意地研究了在人类尿液样品中Bapt-7检测aptasensor的性能。这些发现可能有助于区分这种具有挑战性的疾病，并有助于人类健康。