The Burkholderia cepacia complex is a group of closely related bacterial species with large genomes that infect immunocompromised individuals and those living with cystic fibrosis. Some of these species are found more frequently and cause more severe disease than others, yet metabolomic differences between these have not been described. Furthermore, our understanding of how these species respond to antibiotics is limited. We investigated the metabolomics differences between three most prevalent Burkholderia spp. associated with cystic fibrosis: B. cenocepacia, B. multivorans, and B. dolosa in the presence and absence of the antibiotic trimethoprim. Using a combination of supervised and unsupervised metabolomics data visualization and analysis tools, we describe the overall differences between strains of the same species and between species. Specifically, we report, for the first time, the role of the pyomelanin pathway in the metabolism of trimethoprim. We also report differences in the detection of known secondary metabolites such as fragin, ornibactin, and N-acylhomoserine lactones and their analogs in closely related strains. Furthermore, we highlight the potential for the discovery of new secondary metabolites in clinical strains of Burkholderia spp. The metabolomics differences described in this study highlight the personalized nature of closely related Burkholderia strains.

中文翻译：

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囊性纤维化相关伯克霍尔德氏菌的差异。暴露于抗生素甲氧苄啶后的细菌代谢组。

洋葱伯克霍尔德菌复合体是一组紧密相关的细菌，具有较大的基因组，可感染免疫功能低下的人和患有囊性纤维化的人。这些物种中的一些比其他物种更常见，并导致更严重的疾病，但尚未描述它们之间的代谢组学差异。此外，我们对这些物种对抗生素的反应的理解是有限的。我们调查了三种最普遍的伯克霍尔德氏菌之间的代谢组学差异。与囊性纤维化相关：B。cenocepacia，B。multivorans和B. dolosa在存在和不存在甲氧苄啶的情况下。结合有监督和无监督的代谢组学数据可视化和分析工具，我们描述了同一物种之间以及物种之间的总体差异。具体而言，我们首次报道了吡美拉宁途径在甲氧苄啶的代谢中的作用。我们还报告了在密切相关的菌株中已知的次生代谢产物（例如fragin，鸟氨酸和N-酰基高丝氨酸内酯及其类似物）的检测差异。此外，我们强调了伯克霍尔德氏菌属临床菌株中发现新的次生代谢产物的潜力。在这项研究中描述的代谢组学差异突出了紧密相关的伯克霍尔德菌菌株的个性化性质。我们强调了在伯克霍尔德氏菌属临床菌株中发现新的次生代谢产物的潜力。在这项研究中描述的代谢组学差异突出了紧密相关的伯克霍尔德菌菌株的个性化性质。我们强调了在伯克霍尔德氏菌属临床菌株中发现新的次生代谢产物的潜力。在这项研究中描述的代谢组学差异突出了紧密相关的伯克霍尔德菌菌株的个性化性质。