Peripheral nerve injuries always cause dysfunction but without ideal strategies to assist the treatment and recovery successfully. The primary way to repair the peripheral nerve injuries is to bridge the lesions by promoting axon regeneration. Schwann cells acting as neuroglial cells play a pivotal role during axonal regeneration. The orderly and organized migration of Schwann cells is beneficial for the extracellular matrix connection and Büngner bands formation, which greatly promote the regeneration of axons by offering mechanical support and growth factors. Thus, the use of Schwann cells as therapeutic cells offers us an attractive method for neurorepair therapies, and the ability to direct and manipulate Schwann cell migration and distribution is of great significance in the field of cell therapy in regards to the repair and regeneration of the peripheral nerve. Herein, we design and characterize a type of novel fluorescent–magnetic bifunctional Fe₃O₄·Rhodamine 6G (R6G)@polydopamine (PDA) superparticles (SPs) and systematically study the biological behaviors of Fe₃O₄·R6G@PDA SP uptake by Schwann cells. The results demonstrate that our tailor-made Fe₃O₄·R6G@PDA SPs can be endocytosed by Schwann cells and then highly magnetize Schwann cells by virtue of their excellent biocompatibility. Furthermore, remote-controlling and noninvasive magnetic targeting migration of Schwann cells can be achieved on the basis of the high magnetic responsiveness of Fe₃O₄·R6G@PDA SPs. At the end, gene expression profile analysis is performed to explore the mechanism of Schwann cells’ magnetic targeting migration. The results indicate that cells can sense external magnetic mechanical forces and transduce into intracellular biochemical signaling, which stimulate gene expression associated with Schwann cell migration.

中文翻译：

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雪旺细胞通过荧光-磁性双功能Fe3O4·若丹明6G @聚多巴胺超粒子介导的磁致动迁移。

周围神经损伤通常会导致功能障碍，但没有理想的策略来成功地协助治疗和康复。修复周围神经损伤的主要方法是通过促进轴突再生来桥接病变。充当神经胶质细胞的雪旺细胞在轴突再生过程中起关键作用。雪旺细胞的有序和有组织的迁移有利于细胞外基质连接和邦格纳带的形成，通过提供机械支持和生长因子极大地促进轴突的再生。因此，将雪旺氏细胞用作治疗性细胞为我们提供了一种有吸引力的神经修复疗法，指导和操纵雪旺细胞迁移和分布的能力在细胞治疗领域对周围神经的修复和再生具有重要意义。在这里，我们设计和表征一种新型的荧光-磁性双功能铁₃ O ₄ ·若丹明6G（R6G）@聚多巴胺（PDA）超微粒（SP），并系统研究了Schwann细胞摄取Fe ₃ O ₄ ·R6G @ PDA SP的生物学行为。结果表明，我们量身定制的Fe ₃ O ₄ ·R6G @ PDA SPs可以被雪旺氏细胞内吞，然后由于其优异的生物相容性而高度磁化雪旺氏细胞。此外，基于Fe ₃ O ₄的高磁响应性，可以实现雪旺细胞的远程控制和无创磁性靶向迁移·R6G @ PDA SP。最后，进行基因表达谱分析以探索雪旺细胞的磁性靶向迁移机制。结果表明，细胞可以感知外部磁力，并转化为细胞内生化信号，从而刺激与雪旺细胞迁移相关的基因表达。