High invasion and metastasis are the major obstacles to successful breast cancertherapy. Indocyanine green (ICG), a photosensitizer for photothermal therapy (PTT), shows potent anticancer efficacy when combined with the chemotherapeutic drug doxorubicin (DOX). Human serum albumin (HSA), a biocompatible carrier material, has been successfully used for the delivery of paclitaxel (Abraxane). In addition, there are ICG functional binding regions in HSA. Thus, a smart assembled nanoplatform (DI@HSA NPs) was constructed to achieve the synergistic effects of chemo- photothermal therapy against breast cancer. Compared to free ICG and free DOX, DI@HSA NPs showed satisfactory stability and exhibited an enhanced tumor targeting capacity. The mild hyperthermia generated by DI@HSA NPs can not only cause tumor photothermal ablation and promote the uptake of DI@HSA NPs by 4T1 cells, but also protect the healthy tissues nearby the tumor from overheating injury. More importantly, DI@HSA NPs greatly amplified the infiltration of CD4⁺ T cells and CD8⁺ T cells, resulting in inhibited tumor growth and metastasis. DI@HSA NPs, as a simple biocompatible nanoagent, showed excellent inhibition of breast cancer growth and metastasis by chemo-photothermal therapy, providing a potential strategy for the future therapy of breast cancer.

中文翻译：

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智能组装的人血清白蛋白纳米载体通过化学光热疗法增强了乳腺癌的治疗和抗肿瘤免疫性。

高度侵袭和转移是成功进行乳腺癌治疗的主要障碍。吲哚菁绿（ICG）是一种用于光热疗法（PTT）的光敏剂，与化疗药物阿霉素（DOX）结合使用时显示出有效的抗癌功效。人血清白蛋白（HSA）是一种生物相容性载体材料，已成功用于紫杉醇（Abraxane）的递送。此外，HSA中还有ICG功能性结合区。因此，构建了智能组装的纳米平台（DI @ HSA NPs）以实现化学光热疗法对乳腺癌的协同作用。与游离的ICG和游离的DOX相比，DI @ HSA NP表现出令人满意的稳定性，并具有增强的肿瘤靶向能力。DI @ HSA NPs产生的轻度高温不仅可以引起肿瘤光热消融，并促进4T1细胞对DI @ HSA NPs的吸收，还可以保护肿瘤附近的健康组织免受过热损伤。更重要的是，DI @ HSA NP大大增强了CD4的浸润⁺ T细胞和CD8 ⁺ T细胞，导致肿瘤生长和转移受到抑制。DI @ HSA NPs是一种简单的生物相容性纳米剂，通过化学光热疗法对乳腺癌的生长和转移具有出色的抑制作用，为将来的乳腺癌治疗提供了潜在的策略。