当前位置: X-MOL 学术Cent. Eur. J. Immunol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Laminarin enhances the activity of natural killer cells in immunosuppressed mice.
Central European Journal of Immunology ( IF 1.3 ) Pub Date : 2020-01-20 , DOI: 10.5114/ceji.2019.92784
Xiaobao Zhu 1 , Renzhong Zhu 1 , Zhiyuan Jian 1 , Hong Yu 2
Affiliation  

The aim of the study was to investigate the effects of laminarin on natural killer (NK) cell cytotoxicity of immunosuppressive mice and its mechanism. Cyclophosphamide (cy) was used to make an immunosuppressive model of mice. The mice of two groups were given interventions by gavage with laminarin 500 mg/kg and 1000 mg/kg every day for 10 days. MACS was adopted to isolate spleen NK cells, and cytotoxicity of NK cells and IL-12, IFN-γ level in serum were detected in vivo. Cytotoxicity of NK92-MI cells, activating receptors (NKp30, NKp44, NKp46 and NKG2D) and perforin and granzyme B expression were detected in vitro. Compared to the normal control group, the cytotoxicity of NK cells, IL-12 and IFN-γ level in serum in the cy model group were all reduced significantly (p < 0.01). Compared to the cy model group, laminarin increased the cytotoxicity of NK cells, IL-12 and IFN-γ levels in serum significantly (p < 0.05). In vitro, laminarin increased the cytotoxicity, NKp30 and NKG2D, perforin and granzyme B expressions of NK92-MI cells (p < 0.01). This research showed that laminarin can promote NK cell cytotoxicity in immunosuppressive mice by increasing the levels of IL-12 and IFN-γ in serum and expressions of NKp30 and NKG2D, perforin and granzyme B.

中文翻译:

层粘连蛋白可增强免疫抑制小鼠的自然杀伤细胞活性。

该研究的目的是研究层粘连蛋白对免疫抑制小鼠的自然杀伤(NK)细胞毒性的作用及其机制。环磷酰胺(cy)用于制作小鼠的免疫抑制模型。每天给两组小鼠灌胃laminarin 500 mg / kg和1000 mg / kg,持续10天。采用MACS分离脾脏NK细胞,在体内检测NK细胞的细胞毒性和血清中IL-12,IFN-γ水平。体外检测NK92-MI细胞,激活受体(NKp30,NKp44,NKp46和NKG2D)的细胞毒性以及穿孔素和颗粒酶B的表达。cy模型组与正常对照组相比,NK细胞的细胞毒性,血清中IL-12和IFN-γ水平均明显降低(p <0.01)。与cy模型组相比,层粘连蛋白显着增加了血清中NK细胞的细胞毒性,IL-12和IFN-γ水平(p <0.05)。在体外,层粘连蛋白可提高NK92-MI细胞的细胞毒性,NKp30和NKG2D,穿孔素和颗粒酶B的表达(p <0.01)。这项研究表明,laminarin可以通过增加血清中IL-12和IFN-γ的水平以及NKp30和NKG2D,穿孔素和颗粒酶B的表达来促进免疫抑制小鼠的NK细胞细胞毒性。
更新日期:2020-01-20
down
wechat
bug