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Expression profile of the carbon reserve remobilization from the source to sink in rice in response to soil drying during grain filling
Food and Energy Security ( IF 5 ) Pub Date : 2020-04-01 , DOI: 10.1002/fes3.204
Guanqun Wang 1, 2 , Haoxuan Li 2 , Yulong Gong 3 , Jianchang Yang 4 , Yake Yi 1 , Jianhua Zhang 2, 3 , Nenghui Ye 1, 2
Affiliation  

Moderate soil drying (MD) applied during the mid‐to‐late grain filling stages can promote carbon reserve remobilization in straws (sheaths and stems) and increase the grain weight. The coordination between starch‐to‐sucrose transition in straws and sucrose‐to‐starch conversion in inferior grains is essential for carbon reserve remobilization during grain filling. Herein, to reveal the regulating mechanism of carbon reserve remobilization from source to sink, RNA‐seq was utilized to analyze the dynamic transcript profile in source and sink of rice under MD treatment during grain filling. The expression of amylase genes and amylase activity was enhanced by MD treatment in straws, which is consistent with the increased amylase activity. In inferior grains, it was starch synthesis genes that were upregulated by MD treatment. Furthermore, an elevated ABA was found in both straws and inferior grains, which was caused by an increased expression of NCED1 and downregulation of ABA8OX2 by MD treatment, respectively. Additionally, the expression of MYB30, a transcription factor (TF) that negatively regulates beta‐amylase genes, was reduced in straws by MD, resulting in an increased amylase activity. In contrast, an increased expression of NAC activated the expressions of starch synthesis gene in inferior grains under MD. Therefore, it is MYB30 and NAC that cooperates in source and sink, respectively, to promote carbon reserve remobilization in response to MD. Taken together, genes involved in carbon flow from source to sink are different between rice straws and inferior grains.

中文翻译:

灌浆过程中土壤干燥导致从源头转移到下沉的碳储量的表达特征

在后期至后期的灌浆阶段进行适度的土壤干燥(MD),可以促进秸秆(鞘和茎)中碳储备的迁移,并增加谷物的重量。秸秆中淀粉向蔗糖的转化与劣质谷物中蔗糖向淀粉的转化之间的协调对于灌浆过程中碳储量的转移至关重要。在本文中,为了揭示碳源从源到库转移的调节机制,利用RNA-seq分析了在灌浆过程中MD处理下水稻源和库的动态转录本分布。秸秆的MD处理提高了淀粉酶基因的表达和淀粉酶活性,这与增加的淀粉酶活性是一致的。在劣等谷物中,淀粉合成基因被MD处理上调。此外,NCED1和下调ABA8OX2由医师治疗,分别。此外,MD降低稻草中MYB30(一种负调节β-淀粉酶基因的转录因子(TF))的表达,导致淀粉酶活性增加。相反,在MD下,NAC的表达增加激活了劣等谷物中淀粉合成基因的表达。因此,MYB30NAC分别在源和汇方面进行合作,以促进应对MD的碳储备迁移。两者合计,稻草和劣质谷物中涉及从源到汇碳流的基因是不同的。
更新日期:2020-04-01
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