In optical coherence tomography (OCT), the axial resolution is often superior to the lateral resolution, which is sacrificed for long imaging depths. To address this anisotropy, we previously developed optical coherence refraction tomography (OCRT), which uses images from multiple angles to computationally reconstruct an image with isotropic resolution, given by the OCT axial resolution. On the other hand, spectroscopic OCT (SOCT), an extension of OCT, trades axial resolution for spectral resolution and hence often has superior lateral resolution. Here, we present spectroscopic OCRT (SOCRT), which uses SOCT images from multiple angles to reconstruct a spectroscopic image with isotropic spatial resolution limited by the OCT lateral resolution. We experimentally show that SOCRT can estimate bead size based on Mie theory at simultaneously high spectral and isotropic spatial resolution. We also applied SOCRT to a biological sample, achieving axial resolution enhancement limited by the lateral resolution.

中文翻译：

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光谱光学相干折射断层扫描

在光学相干断层扫描（OCT）中，轴向分辨率通常优于横向分辨率，这是长距离成像深度所牺牲的。为了解决这种各向异性，我们先前开发了光学相干折射断层扫描（OCRT），该技术使用多个角度的图像来计算以OCT轴向分辨率给出的各向同性分辨率的图像。另一方面，光谱OCT（SOCT）是OCT的扩展，将轴向分辨率换成了光谱分辨率，因此通常具有更好的横向分辨率。在这里，我们介绍了光谱OCRT（SOCRT），它使用来自多个角度的SOCT图像重建了各向同性空间分辨率受OCT横向限制的光谱图像解析度。我们通过实验证明，SOCRT可以基于Mie理论同时在高光谱和各向同性空间分辨率下估算磁珠尺寸。我们还将SOCRT应用于生物样品，实现了轴向分辨率增强（受横向分辨率限制）。