This study was performed to investigate the effect of the chloroform fraction from Actinidia arguta (CFAA) on cognitive dysfunction in a C57BL/6 mouse model fed a high-fat diet (HFD) for 12 weeks. The CFAA has the protective effect on high glucose-induced neurotoxicity in MC-IXC cell (neuroblastoma cell line). In a C57BL/6 mouse model fed a HFD for 12 weeks, the improved glucose tolerance and cognitive dysfunction were observed in a group ingesting CFAA. In the brain tissue analysis, the impaired cholinergic, antioxidant system and mitochondria functions were improved in the CFAA group. In addition, in a molecular biology study, it was observed that CFAA improves HFD-induced abnormal insulin signaling such as increase of IRS phosphorylation at serine residues and reduction of Akt phosphorylation caused by the increase of JNK phosphorylation and then inhibited apoptosis. In the UPLC Q-TOF/MS analysis, pentacyclic triterpenoids such as asiatic acid (AA), madecassic acid (MA) were identified in CFAA as main compounds. Therefore, these results propose that Actinidia arguta rich in pentacyclic triterpenoids may be effective as preventive matter a therapeutic strategy to improve neurodegenerative disease caused by HFD.

中文翻译：

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Hardy猕猴桃（Actinidia arguta）富含五环三萜的成分可改善高脂饮食诱导的肥胖小鼠的脑功能障碍。

进行这项研究的目的是，在高脂饮食（HFD）喂养12周的C57BL / 6小鼠模型中，研究猕猴桃氯仿组分（CFAA）对认知功能障碍的影响。CFAA对高糖诱导的MC-IXC细胞（神经母细胞瘤细胞系）神经毒性具有保护作用。在喂食HFD 12周的C57BL / 6小鼠模型中，在摄入CFAA的组中观察到了改善的葡萄糖耐量和认知功能障碍。在脑组织分析中，CFAA组改善了胆碱能，抗氧化系统和线粒体功能的受损。此外，在分子生物学研究中，观察到CFAA改善了HFD诱导的异常胰岛素信号传导，例如丝氨酸残基的IRS磷酸化的增加和由JNK磷酸化的增加引起的Akt磷酸化的减少，然后抑制了细胞凋亡。在UPLC Q-TOF / MS分析中，CFAA中鉴定出五环三萜类化合物（例如积雪草酸（AA），马cas酸（MA））为主要化合物。因此，这些结果表明，富含五环三萜的猕猴桃可以作为预防物质有效地改善由HFD引起的神经变性疾病的治疗策略。