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Production of a specific monoclonal antibody for 1-naphthol based on novel hapten strategy and development of an easy-to-use ELISA in urine samples.
Ecotoxicology and Environmental Safety ( IF 6.8 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.ecoenv.2020.110533
Zi-Jian Chen 1 , Xi-Xia Liu 2 , Zhi-Li Xiao 1 , Hui-Jun Fu 1 , Yu-Ping Huang 1 , Shu-Yi Huang 1 , Yu-Dong Shen 1 , Fan He 1 , Xing-Xing Yang 3 , Bruce Hammock 4 , Zhen-Lin Xu 1
Affiliation  

1-naphthol (1-NAP) is the main metabolite of pesticide carbaryl and naphthalene, and is also a genotoxic and carcinogenic intermediate in the synthesis of organic compound, dyes, pigment and pharmaceutical industry. In this work, two novel haptens were designed and synthesized for developing a competitive indirect enzyme-linked immunosorbent assay (ciELISA) method for 1-NAP in urine samples. The assay showed a limit of detection of 2.21 ng/mL and working range from 4.02 ng/mL to 31.25 ng/mL for 1-NAP in optimized working buffer. The matrix effect of samples was eliminated via 15-fold dilution of optimized working buffer. Good average recoveries (102.4%-123.4%) with a coefficient of variation from 11.7% to 14.7% was obtained for spiked urine samples. Subsequent instrument verification test showed good correlation between the results of ciELISA and high-performance liquid chromatography. The developed ciELISA is a high-throughput tool to monitor 1-NAP in urine, which can provide technical support for the establishment of biological exposure level for the exposure to carbaryl, naphthalene and other related pollutants.

中文翻译:

基于新型半抗原策略的1-萘酚特异性单克隆抗体的生产以及尿液样品中易于使用的ELISA的开发。

1-萘酚(1-NAP)是农药西维因和萘的主要代谢产物,也是有机化合物,染料,颜料和制药工业合成中的遗传毒性和致癌中间体。在这项工作中,设计并合成了两种新型的半抗原,用于开发竞争性间接酶联免疫吸附测定(ciELISA)方法用于尿液样品中的1-NAP。该测定显示优化的工作缓冲液中1-NAP的检测限为2.21 ng / mL,工作范围为4.02 ng / mL至31.25 ng / mL。通过优化工作缓冲液的15倍稀释消除了样品的基质效应。对于加标尿液样品,获得了良好的平均回收率(102.4%-123.4%),变异系数从11.7%到14.7%。随后的仪器验证测试表明ciELISA结果与高效液相色谱法之间具有良好的相关性。发达的ciELISA是监测尿液中1-NAP的高通量工具,可为建立暴露于西维因,萘和其他相关污染物的生物暴露水平提供技术支持。
更新日期:2020-04-01
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