Perchloroethene (PCE) is a hazardous and persistent groundwater pollutant. Both treatment with nanoscaled zero-valent iron (nZVI) and biological degradation by bacteria have downsides. Distribution of nZVI underground is difficult and a high percentage of injected nZVI is consumed by anaerobic corrosion, forming H2 rather than being available for PCE dechlorination. On the other hand, microbial PCE degradation can suffer from the absence of H2. This can cause the accumulation of the hazardous metabolites cis-1,2-dichloroethene (DCE) or vinylchloride (VC). The combination of chemical and biological PCE degradation is a promising approach to overcome the disadvantages of each method alone. In this lysimeter study, artificial aquifers were created to test the influence of nZVI on anaerobic microbial PCE dechlorination by a commercially available culture containing Dehalococcoides spp under field-like conditions. The effect of the combined treatment was investigated with molasses as an additional electron source and after cessation of molasses addition. The combination of nZVI and the Dehalococcoides spp. containing culture led to a PCE discharge in the lysimeter outflow that was 4.7 times smaller than that with nZVI and 1.6 times smaller than with bacterial treatment. Moreover, fully dechlorinated end-products showed an 11-fold increase compared to nZVI and a 4.2-fold increase compared to the microbial culture. The addition of nZVI to the microbial culture also decreased the accumulation of hazardous metabolites by 1.7 (cis-DCE) and 1.2 fold (VC). The stimulatory effect of nZVI on microbial degradation was most obvious after the addition of molasses was stopped.

中文翻译：

---

全氯乙烯和纳米零价铁 (nZVI) 微生物厌氧脱氯的协同效应——蒸渗仪实验

全氯乙烯 (PCE) 是一种危险且持久的地下水污染物。纳米零价铁 (nZVI) 处理和细菌生物降解都有缺点。nZVI 在地下的分布很困难，而且大部分注入的 nZVI 被厌氧腐蚀消耗，形成 H2 而不能用于 PCE 脱氯。另一方面，微生物 PCE 降解会因缺乏 H2 而受到影响。这会导致有害代谢物 cis-1,2-dichloroethene (DCE) 或氯乙烯 (VC) 的积累。化学和生物 PCE 降解的结合是克服单独每种方法的缺点的有前途的方法。在这项蒸渗仪研究中，创建了人工含水层，以测试 nZVI 对厌氧微生物 PCE 脱氯的影响，这种方法是在田间条件下通过市售的含有 Dehalococcoides spp 的培养物进行的。用糖蜜作为额外的电子源并在糖蜜添加停止后研究组合处理的效果。nZVI 和 Dehalococcoides spp 的组合。含培养物导致蒸渗仪流出物中的 PCE 排放量比 nZVI 小 4.7 倍，比细菌处理小 1.6 倍。此外，完全脱氯的最终产品与 nZVI 相比增加了 11 倍，与微生物培养相比增加了 4.2 倍。将 nZVI 添加到微生物培养物中也将有害代谢物的积累减少了 1.7 (cis-DCE) 和 1.2 倍 (VC)。