Despite the daily consumption of copper chlorophylls (E-141i), the green food colorants in foods high in fats, there is a general need for knowledge regarding their exact composition. Consequently, we have analyzed by HPLC-ESI(+)/APCI(+)-hrTOF-MS2 the accurate composition of different commercial copper chlorophyll colorants for the first time. Data showed a favored yield of copper pheophytins from a series, while pheophytins from b series are preferentially no complexed with copper. The copper pheophytins present in the food colorants consisted mainly of three structural rearrangements. New fragmentation patterns and structural assignments have been described for several copper pheophytins. During the ingestion of copper chlorophylls, no chlorophyll derivative was present in serum nor urine except a new copper-pyroporphyrin a accumulated in a few livers. In any case, this green additive could represent the ideal food colorant, as most of the copper pheophytins are excreted in the feces showing almost no absorption of copper-chlorophylls compounds.

中文翻译：

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铜叶绿素的HPLC-hrTOF-MS研究：食用色素的成分和摄入后的生物化学。

尽管每天食用高脂肪食品中的绿色食品着色剂铜叶绿素（E-141i），但通常需要有关其确切成分的知识。因此，我们首次通过HPLC-ESI（+）/ APCI（+）-hrTOF-MS2分析了不同商业铜叶绿素着色剂的准确组成。数据显示，一系列铜脱镁叶绿素的收率较高，而b系列脱镁叶绿素优选不与铜形成络合物。食品色素中存在的铜脱镁叶绿素主要由三个结构重排组成。已经描述了几种铜脱镁叶绿素的新的断裂模式和结构分配。在摄入叶绿素铜的过程中，血清和尿液中均不存在叶绿素衍生物，只有少数肝脏中积累了新的铜卟啉。在任何情况下，这种绿色添加剂都可以代表理想的食用色素，因为大多数铜脱镁叶绿素从粪便中排出，几乎不吸收铜叶绿素化合物。