Lindane is a highly toxic organochlorine pesticide and widely exist in water with harmful effects on fish. Although some genes have been found to be regulated by lindane in fish, the transcriptional response of fish exposed to lindane is still unknown. In this research, the transcriptional changes of zebrafish larvae exposed to 0.2 mg/L lindane from 96 to 120 hpf were studied by RNA sequencing. Our transcriptome identified 554 up-regulated and 118 down-regulated genes and the differentially expressed genes were closely related to the neuromast development, RNA silencing genes, ion transport, and response to estrogen. In addition, we characterized two sensitive and novel lindane-induced ABCG (ATP binding cassette G subfamily) transporter genes- abcg5 and abcg8. Abcg5 and abcg8 genes are located on chromosome 13 of zebrafish and contain 1956/2024 bp open reading frame. The polypeptide deduced by CDS amplification contains 652/676 amino acids and has most of the functional domains and key residues defined in human and mouse ABCG5/Abcg5 or ABCG8/Abcg8. Only when the co-expression of Abcg5 and Abcg8 enable them to transport to the cell membrane surface in 293T cells. In addition, lindane can induce the transcriptional expression of abcg5 and abcg8 genes, and overexpression of Abcg5 and Abcg8 significantly reduced the toxicity of lindane to zebrafish larvae, which means that zebrafish Abcg5 and Abcg8 are potential efflux transporters of lindane. Therefore, these findings provide useful insights for further understanding the zebrafish larvae's transcriptional response and detoxification ability after acute exposure to lindane.

中文翻译：

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暴露于林丹的斑马鱼幼虫的转录反应揭示了ABC转运蛋白家族的两个排毒基因（abcg5和abcg8）。

林丹是一种剧毒的有机氯农药，广泛存在于水中，对鱼类有害。尽管已发现某些基因受鱼中林丹的调控，但仍不清楚暴露于林丹的鱼的转录反应。在这项研究中，通过RNA测序研究了暴露于0.2 mg / L林丹的斑马鱼幼虫从96到120 hpf的转录变化。我们的转录组确定了554个上调基因和118个下调基因，差异表达的基因与神经肥大发育，RNA沉默基因，离子转运和对雌激素的反应密切相关。此外，我们表征了两个敏感的新型林丹诱导的ABCG（ATP结合盒G亚家族）转运蛋白基因abcg5和abcg8。Abcg5和abcg8基因位于斑马鱼的13号染色体上，并包含1956/2024 bp开放阅读框。通过CDS扩增推导的多肽包含652/676个氨基酸，并具有人类和小鼠ABCG5 / Abcg5或ABCG8 / Abcg8中定义的大多数功能域和关键残基。仅当Abcg5和Abcg8共表达时，它们才能转运到293T细胞的细胞膜表面。此外，林丹可以诱导abcg5和abcg8基因的转录表达，而过表达Abcg5和Abcg8则大大降低了林丹对斑马鱼幼虫的毒性，这意味着斑马鱼Abcg5和Abcg8是林丹的潜在外向转运蛋白。因此，这些发现为进一步了解斑马鱼的幼虫提供了有益的见解。