Abstract 2-phenylethanol (2-PE) is a higher alcohol widely used in industry that can be obtained by solid-state fermentation (SSF) using low-cost raw materials. This report describes the 2-PE production potential of an indigenous Pichia kudriavzevii isolated from solid-state fermented sugarcane bagasse that possesses attractive characteristics for processing waste streams such as its low-pH tolerance, high growth rate and temperature resistance. Besides, 2-PE production was optimized in batch-SSF using sugarcane bagasse supplemented with l -phenylalanine as substrate. Full factorial design allowed identifying the pH adjustment, micronutrient addition, inoculum and co-substrate load effects, and response surface methodology served to identify the maximum production based on temperature, initial moisture content (MC0) and specific airflow rate (SAFR). While the pH adjustment and micronutrient addition did not affect the 2-PE production, temperature and MC0 resulted critical for the process. After optimization, the maximum 2-PE content was 27.2 ± 0.2 mg per gram of dry substrate at 31 °C, 76 % MC0 and 0.129 L h−1 g−1 SAFR. This result was 23.8 % higher than the sub-optimal condition, and it is the highest 2-PE production via SSF reported so far. These results confirm the ability of P. kudriavzevii for producing 2-PE, and its potential for using waste streams as substrate.

中文翻译：

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通过固态发酵分离的 Pichia kudriavzevii 的 2-苯基乙醇（玫瑰香气）生产潜力

摘要 2-苯基乙醇（2-PE）是一种工业上广泛使用的高级醇，可以使用低成本的原料通过固态发酵（SSF）获得。本报告描述了从固态发酵甘蔗渣中分离的本土毕赤酵母 kudriavzevii 的 2-PE 生产潜力，该毕赤酵母具有处理废物流的有吸引力的特性，例如其低 pH 耐受性、高增长率和耐高温性。此外，使用补充有 l-苯丙氨酸作为底物的甘蔗渣在分批 SSF 中优化了 2-PE 生产。全因子设计允许确定 pH 值调整、微量营养素添加、接种物和共底物负载效应，响应面方法用于确定基于温度的最大产量，初始水分含量 (MC0) 和比气流率 (SAFR)。虽然 pH 值调整和微量营养素添加不会影响 2-PE 的生产，但温度和 MC0 对过程至关重要。优化后，在 31 °C、76% MC0 和 0.129 L h-1 g-1 SAFR 下，最大 2-PE 含量为每克干底物 27.2 ± 0.2 mg。该结果比次优条件高 23.8%，是迄今为止通过 SSF 报告的最高 2-PE 产量。这些结果证实了 P. kudriavzevii 生产 2-PE 的能力，及其使用废物流作为底物的潜力。129 L h-1 g-1 SAFR。该结果比次优条件高 23.8%，是迄今为止通过 SSF 报告的最高 2-PE 产量。这些结果证实了 P. kudriavzevii 生产 2-PE 的能力，及其使用废物流作为底物的潜力。129 L h-1 g-1 SAFR。该结果比次优条件高 23.8%，是迄今为止通过 SSF 报告的最高 2-PE 产量。这些结果证实了 P. kudriavzevii 生产 2-PE 的能力，及其使用废物流作为底物的潜力。