The boost of research output in lipidomics during the last decade is tightly linked to improved instrumentation in mass spectrometry. Associated with this trend is the shift from low resolution—toward high‐resolution lipidomics platforms. This review article summarizes the state of the art in the lipidomics field with a particular focus on the merits of high mass resolution. Following some theoretical considerations on the benefits of high mass resolution in lipidomics, it starts with a historical perspective on lipid analysis by sector instruments and moves further to today's instrumental approaches, including shotgun lipidomics, liquid chromatography–mass spectrometry, matrix‐assisted laser desorption ionization‐time‐of‐flight, and imaging lipidomics. Subsequently, several data processing and data analysis software packages are critically evaluated with all their pros and cons. Finally, this article emphasizes the importance and necessity of quality standards as the field evolves from its pioneering phase into a mature and robust omics technology and lists various initiatives for improving the applicability of lipidomics. © 2020 The Authors. Mass Spectrometry Reviews published by John Wiley & Sons Ltd. Mass Spec Rev

中文翻译：

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脂质体中的高分辨率质谱

过去十年脂质组学研究成果的增长与质谱仪器的改进密切相关。与这一趋势相关的是从低分辨率向高分辨率脂质组学平台的转变。这篇评论文章总结了脂质组学领域的最新技术，特别关注高质量分辨率的优点。在对脂质组学中高质量分辨率的好处进行一些理论考虑之后，它从部门仪器脂质分析的历史视角开始，并进一步转向当今的仪器方法，包括鸟枪脂质组学、液相色谱-质谱法、基质辅助激光解吸电离飞行时间和成像脂质组学。随后，几个数据处理和数据分析软件包都经过严格评估，并确定了它们的所有优点和缺点。最后，本文强调了质量标准的重要性和必要性，因为该领域从开创阶段发展为成熟而强大的组学技术，并列出了提高脂质组学适用性的各种举措。© 2020 作者。由 John Wiley & Sons Ltd. 出版的质谱评论。 Mass Spec Rev