In an ultrasonic bath and for 30 min in the presence of water as extraction solvent in a ratio of 1:10 (g/mL), effect of extraction temperature on extraction of caffeoylquinic acids (CQAs) from Moringa oleifera leaves (MOL) was investigated. Gradually increasing in extraction temperature caused a growing in mono‐CQA and declining in 3,5‐diCQA. The extract obtained at 80 °C showed the highest antioxidant capacity, and IC₅₀ values for scavenging activities on DPPH radicals and ABTS radicals were 56.9 and 53.6 µg/mL, respectively. Compared with α‐glucosidase and α‐amylase, the extract showed the highest inhibition on pancreatic lipase (IC₅₀ of 0.073 mg/mL). Increasing the extraction temperature improved cytotoxicity of the extract on cancer cell lines of HeLa and HepG2. Compared with gram‐negative Escherichia coli and Salmonella typhimurium, all the extracts showed higher inhibition on the growth of gram‐positive Bacillus cereus and Staphylococcus aureus. Our results suggested that use of higher extraction temperature (80 °C) improved the biological activity of MOL extract.

中文翻译：

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浸渍温度对辣木叶中咖啡酰奎尼酸衍生物的提取及其对消化酶，抗氧化剂，抗增殖和抑菌活性的抑制作用

在超声浴中，在水为萃取溶剂的比例为1:10（g / mL）的条件下放置30分钟，研究了萃取温度对辣木叶（MOL）提取咖啡酰奎尼酸（CQAs）的影响。提取温度的逐渐升高导致mono-CQA的增加，而3,5-diCQA的下降。在80°C下获得的提取物具有最高的抗氧化能力，清除DPPH自由基和ABTS自由基的IC ₅₀值分别为56.9和53.6 µg / mL。与α-葡萄糖苷酶和α-淀粉酶相比，提取物对胰腺脂肪酶的抑制作用最大（IC ₅₀0.073 mg / mL）。提高提取温度可提高提取物对HeLa和HepG2癌细胞系的细胞毒性。与革兰氏阴性大肠杆菌和鼠伤寒沙门氏菌相比，所有提取物均对革兰氏阳性蜡状芽孢杆菌和金黄色葡萄球菌的生长具有更高的抑制作用。我们的结果表明，使用较高的提取温度（80°C）可以改善MOL提取物的生物活性。