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The cyanobacterial phytochrome 2 regulates the expression of motility-related genes through the second messenger cyclic di-GMP.
Photochemical & Photobiological Sciences ( IF 3.1 ) Pub Date : 2020-04-07 , DOI: 10.1039/c9pp00489k Thomas Wallner 1 , Laura Pedroza 1 , Karsten Voigt 1 , Volkhard Kaever 2 , Annegret Wilde 1
Photochemical & Photobiological Sciences ( IF 3.1 ) Pub Date : 2020-04-07 , DOI: 10.1039/c9pp00489k Thomas Wallner 1 , Laura Pedroza 1 , Karsten Voigt 1 , Volkhard Kaever 2 , Annegret Wilde 1
Affiliation
The cyanobacterial phytochrome Cph2 is a light-dependent diguanylate cyclase of the cyanobacterium Synechocystis 6803. Under blue light, Cph2-dependent increase in the cellular c-di-GMP concentration leads to inhibition of surface motility and enhanced flocculation of cells in liquid culture. However, the targets of second messenger signalling in this cyanobacterium and its mechanism of action remained unclear. Here, we determined the cellular concentrations of cAMP and c-di-GMP in wild-type and Δcph2 cells after exposure to blue and green light. Inactivation of cph2 completely abolished the blue-light dependent increase in c-di-GMP content. Therefore, a microarray analysis with blue-light grown wild-type and Δcph2 mutant cells was used to identify c-di-GMP dependent alterations in transcript accumulation. The increase in the c-di-GMP content alters expression of genes encoding putative cell appendages, minor pilins and components of chemotaxis systems. The mRNA encoding the minor pilins pilA5-pilA6 was negatively affected by high c-di-GMP content under blue light, whereas the minor pilin encoding operon pilA9-slr2019 accumulates under these conditions, suggesting opposing functions of the respective gene sets. Artificial overproduction of c-di-GMP leads to similar changes in minor pilin gene expression and supports previous findings that c-di-GMP is important for flocculation via the function of minor pilins. Mutational and gene expression analysis further suggest that SyCRP2, a CRP-like transcription factor, is involved in regulation of minor pilin and putative chaperone usher pili gene expression.
中文翻译:
蓝细菌植物色素2通过第二信使环di-GMP调节与运动相关的基因的表达。
蓝细菌植物色素Cph2是蓝细菌Synechocystis 6803的一种光依赖性双鸟苷酸环化酶。在蓝光下,细胞c-di-GMP浓度中Cph2依赖性增加导致液体培养中细胞表面运动性的增强和细胞絮凝的增强。但是,该蓝细菌中第二信使信号的靶标及其作用机理仍不清楚。在这里,我们确定了暴露于蓝光和绿光后野生型和Δcph2细胞中cAMP和c-di-GMP的细胞浓度。cph2的灭活完全消除了c-di-GMP含量的蓝光依赖性增加。因此,使用蓝光生长的野生型和Δcph2突变细胞进行的微阵列分析可用于识别转录本积累中c-di-GMP依赖性变化。c-di-GMP含量的增加会改变编码假定的细胞附件,次要菌毛素和趋化性系统组分的基因的表达。编码次要菌毛pilA5-pilA6的mRNA在蓝光下受到高c-di-GMP含量的负面影响,而次要菌毛编码操纵子pilA9-slr2019则在这些条件下积累,表明各个基因集的功能相反。人工过量生产c-di-GMP会导致次要菌毛蛋白基因表达发生类似变化,并支持先前的发现,即c-di-GMP通过次要菌毛蛋白的功能对于絮凝很重要。突变和基因表达分析进一步表明,SyCRP2(一种CRP样转录因子)参与调节小菌毛蛋白和推定的伴侣分子引入菌毛基因的表达。
更新日期:2020-03-31
中文翻译:
蓝细菌植物色素2通过第二信使环di-GMP调节与运动相关的基因的表达。
蓝细菌植物色素Cph2是蓝细菌Synechocystis 6803的一种光依赖性双鸟苷酸环化酶。在蓝光下,细胞c-di-GMP浓度中Cph2依赖性增加导致液体培养中细胞表面运动性的增强和细胞絮凝的增强。但是,该蓝细菌中第二信使信号的靶标及其作用机理仍不清楚。在这里,我们确定了暴露于蓝光和绿光后野生型和Δcph2细胞中cAMP和c-di-GMP的细胞浓度。cph2的灭活完全消除了c-di-GMP含量的蓝光依赖性增加。因此,使用蓝光生长的野生型和Δcph2突变细胞进行的微阵列分析可用于识别转录本积累中c-di-GMP依赖性变化。c-di-GMP含量的增加会改变编码假定的细胞附件,次要菌毛素和趋化性系统组分的基因的表达。编码次要菌毛pilA5-pilA6的mRNA在蓝光下受到高c-di-GMP含量的负面影响,而次要菌毛编码操纵子pilA9-slr2019则在这些条件下积累,表明各个基因集的功能相反。人工过量生产c-di-GMP会导致次要菌毛蛋白基因表达发生类似变化,并支持先前的发现,即c-di-GMP通过次要菌毛蛋白的功能对于絮凝很重要。突变和基因表达分析进一步表明,SyCRP2(一种CRP样转录因子)参与调节小菌毛蛋白和推定的伴侣分子引入菌毛基因的表达。