Virus resistance genes carried by wild plant species are valuable resources for plant breeding. The Rysto gene, conferring a broad spectrum of durable resistance, originated from Solanum stoloniferum and was introgressed into several commercial potato cultivars, including 'White Lady', by classical breeding. Rysto was mapped to chromosome XII in potato, and markers used for marker-assisted selection in breeding programmes were identified. Nevertheless, there was no information on the identity of the Rysto gene. To begin to reveal the identification of Rysto, fine-scale genetic mapping was performed which, in combination with chromosome walking, narrowed down the locus of the gene to approximately 1 Mb. DNA sequence analysis of the locus identified six full-length NBS-LRR-type (short NLR-type) putative resistance genes. Two of them, designated TMV2 and TMV3, were similar to a TMV resistance gene isolated from tobacco and to Y-1, which co-segregates with Ryadg, the extreme virus resistance gene originated from Solanum andigena and localised to chromosome XI. Furthermore, TMV2 of 'White Lady' was found to be 95% identical at the genomic sequence level with the recently isolated Rysto gene of the potato cultivar 'Alicja'. In addition to the markers identified earlier, this work generated five tightly linked new markers which can serve potato breeding efforts for extreme virus resistance.

中文翻译：

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Rysto基因座的定位和DNA序列表征赋予了对马铃薯栽培品种“ White Lady”的极强病毒抗性。

野生植物物种携带的抗病毒基因是植物育种的宝贵资源。Rysto基因具有广泛的持久抗性，起源于茄子茄（Solanum stoloniferum），并通过经典育种渗入包括“ White Lady”在内的几种商业马铃薯品种。Rysto被定位到马铃薯的XII染色体上，并鉴定了在育种程序中用于标记辅助选择的标记。但是，没有关于Rysto基因身份的信息。为了开始揭示Rysto的身份，我们进行了精细的基因定位，并与染色体行走相结合，将基因的基因位点缩小到大约1 Mb。该基因座的DNA序列分析确定了六个全长NBS-LRR型（短NLR型）推定抗性基因。他们两个人，分别命名为TMV2和TMV3的TMV与从烟草中分离的TMV抗性基因以及与Ryadg共同分离的Y-1相似，Ryadg是源自茄和茄的极端病毒抗性基因，并位于XI染色体上。此外，发现“白夫人”的TMV2在基因组序列水平上与马铃薯品种“ Alicja”的最近分离的Rysto基因具有95％的同一性。除了较早发现的标记外，这项工作还产生了五个紧密相连的新标记，这些标记可为马铃薯育种工作提供极高的抗病毒性。发现在基因组序列水平上与马铃薯分离品种'Alicja'的Rysto基因在基因组序列水平上具有95％的同一性。除了较早发现的标记外，这项工作还产生了五个紧密相连的新标记，这些标记可为马铃薯育种工作提供极高的抗病毒性。发现在基因组序列水平上与马铃薯分离品种'Alicja'的Rysto基因在基因组序列水平上具有95％的同一性。除了较早发现的标记外，这项工作还产生了五个紧密相连的新标记，这些标记可为马铃薯育种工作提供极高的抗病毒性。